腺苷A1和A3受体参与的DRG神经元内Ca^2＋释放的多光子成像  

Multiphoton imaging of A_1 and A_3 receptors mediating calcium dynamics induced by adenosine in DRG neurons

作　　者：陈江旭[1] 郑莉琴[1] 何逸鹏 杨洪钦[1] 李晖[1] 谢树森[1] 

机构地区：[1]福建师范大学激光与光电子技术研究所 医学光电科学与技术教育部重点实验室 福建省光子技术重点实验室,福建福州350007

出　　处：《光电子．激光》2014年第4期811-817,共7页Journal of Optoelectronics·Laser

基　　金：国家自然科学基金(61335011);长江学者和创新团队发展计划(IRT1115)资助项目

摘　　要：运用激光共聚焦显微成像技术,通过应用腺苷受体的特异性阻断剂和激动剂,研究腺苷受体对腺苷诱导的大鼠背根神经节(DRG)神经元中Ca2+的动态变化的影响,并且进一步研究了腺苷诱导Ca2+动态变化的剂量相关性。结果显示,腺苷A1和A3受体参与腺苷诱导的DRG神经元Ca2+释放,腺苷能引起DRG神经元内Ca2+浓度升高且呈剂量相关性。研究结果表明,共焦显微成像能有效检测单细胞内离子的动态信号,有助于了解腺苷及其受体参与疼痛过程中细胞离子信号传递的机制。Adenosine receptors have been further subdivided into four subtypes, A1, A2A, A2B and A3, all of which couple to G proteins and have been cloned successfully. In order to investigate which adenosine receptors are involved in adenosine-induced cellular Ca2＋ dynamics in dorsal root ganglion （DRG） neurons and whether the effects of adenosine on intracdlular Ca2＋ dynamics are dose-response,in this study we use selective agonists and antagonists of adenosine receptors by laser scanning confocal microscopy and Ca2＋ fluorescent dye Fluo 4/AM. Our results show that using adenosine A1 and A3 receptors selective antagonists could block adenosine-induced Ca2＋ dynamies, which is not found with adenosine A2A and A2B receptors selective antagonists. It shows that adenosine A1 receptor （A1 R） and adenosine A3 receptor （AaR） might be related to adenosine-evoked Ca2＋ release in DRG neurons. In addition,we find that the adenosine stimulation of intracellular Ca2＋ concentration in DRG neurons is dose-response. This study suggests that the cellular ion signaling could be detected efficiently by laser scanning confocal microscopy. The results are helpful to reveal the role of adenosine and adenosine receptor in ion signal transmission during pain.

关 键 词：腺苷受体 CA^2+ 背根神经节(DRG)神经元 激光共聚焦显微成像 

分 类 号：R318.51[医药卫生—生物医学工程]