甘草配伍大戟的体外肝毒性研究  

In vitro hepatotoxicity of compatibility of Glycyrrhizae Radix and Euphorbiae Pekinensis Radix

作　　者：吴秀稳[1] 彭玉帅[1] 王如峰[1] 刘莉嘉 杨鑫[1] 张陆军[2] 刘辰[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国医药集团总公司,北京100191

出　　处：《药物评价研究》2014年第2期122-125,共4页Drug Evaluation Research

基　　金：北京中医药大学自主选题项目"大戟的主要化学成分及其人肠内菌转化研究"

摘　　要：目的研究甘草和大戟配伍的体外肝毒性。方法采用显微观察法和MTT法检测不同浓度的甘草单煎液、大戟单煎液、甘草-大戟合煎液和甘草-大戟单煎混合液对人肝癌细胞HepG2增殖的影响,并比较大戟单煎液、甘草-大戟合煎液和甘草-大戟单煎混合液相当浓度下细胞毒性的大小。结果大戟单用及大戟与甘草配伍均有细胞毒性,且呈剂量相关性;与大戟单煎液相比,甘草-大戟单煎混合液细胞毒性无明显差异,甘草-大戟合煎液细胞毒性减小。结论甘草和大戟配伍导致大戟的体外肝毒性减小。Objeetive To study the hepatotoxicity of compatibility of Glycyrrhizae Radix （GR） and Euphorbiae Pekinensis Radix （EPR） in vitro. Methods Microscopic observation and MTT assay were employed to evaluate the effects of GR, EPR, GR-EPR decoction, and GR-EPR mixture at different concentration on the proliferation of human hepatoma cell line HepG-2, and their cytotoxicities were compared. Results Both EPR and compatibility of GR-EPR exhibited the inhibitory effect on cell proliferation in a dose-dependent manner. Compared with EPR, GR-EPR decoction showed lower cytotoxicity, while the GR-EPR mixture was almost as cytotoxic as EPR. Conclusion Compatibility of GR and EPR could reduce the hepatotoxicity.

关 键 词：甘草 大戟 肝毒性 MTT 

分 类 号：R252[医药卫生—中医内科学]