毛果一枝黄花化学成分及其抗炎活性研究  

Chemical constituents of Solidago virgaurea and their anti-inflammatory activities

作　　者：李涛[1,2] 白虹[1,2] 仲浩[1,2] 邓志鹏[1,2] 杜勇[1,2] 姚庆强[1,2] 

机构地区：[1]山东省医学科学院药物研究所 [2]济南大学 山东省医学科学院医学与生命科学学院/山东省罕少见病重点实验室,山东济南250062

出　　处：《中草药》2014年第6期749-754,共6页Chinese Traditional and Herbal Drugs

基　　金：山东省泰山学者-药学特聘专家专项建设工程经费资助

摘　　要：目的对毛果一枝黄花Solidago virgaurea全草的化学成分和抗炎活性进行研究。方法采用硅胶柱色谱、凝胶柱色谱、ODS反相柱色谱及重结晶等方法分离纯化,通过理化常数和波谱分析鉴定化合物结构,并采用ELISA法对得到的苯甲酸苄酯类化合物进行抗炎活性研究。结果从毛果一枝黄花全草95%乙醇提取物中分离得到9个化合物,分别鉴定为2′-甲氧基苯甲醇-2-甲氧基-6-羟基苯甲酸酯(1)、2′-甲氧基苯甲醇-2,6-二甲氧基苯甲酸酯(2)、毛果一枝黄花素(3)、十六烷酸(4)、水杨酸(5)、对羟基苯甲酸(6)、毛果一枝黄花糖苷(7)、山柰酚-3-O-β-D-芸香糖苷(8)、芦丁(9)。结论化合物3和7为新化合物;化合物4和6为首次从一枝黄花属植物中分离得到,化合物1和2为首次从该植物中分离得到。抗炎活性研究表明,化合物1和2对脂多糖(LPS)诱导的小鼠单核细胞RAW 264.7释放的肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)具有抑制作用,而化合物7无明显抑制作用。Objective To study the chemical constituents ofSolidago virgaurea and their pharmacological activities. Methods The compounds were isolated and purified from the plant with chromatography techniques and the chemical structures were identified on the basis of spectrascopic analyses and physicochemical properties, and the anti-inflammatory effects of obtained benzyl benzoate compounds were evaluated by ELISA. Results Nine compounds were isolated from 90% ethanol extract of S. virgaurea and their structures were identified as 2＇-methoxybenzyl-2-methoxy-6-hydroxybenzoate （1）, 2＇-methoxybenzyl-2, 6-dimethoxybenzoate （2）, solidagobenzofuran （3）, hexadecanoic acid （4）, salicylic acid （5）, p-hydroxybenzoic acid （6）, solidago virgaurea glycoside （7）, kaempferol-3-O-β-D-rutinoside （8）, and rutin （9）. Conclusion Compounds 3 and 7 are new compounds; Compounds 4 and 6 are obtained from the plants of Solidago L. for the first time; Compounds 1 and 2 are isolated from this species for the first time. Anti-inflammatory studies show that compounds 1 and 2 could inhibit the TNF-α and IL-6 release of LPS-induced RAW264.7 murine monocytes, while compound 7 shows no significant inhibitory effect.

关 键 词：毛果一枝黄花 毛果一枝黄花素 毛果一枝黄花糖苷 对羟基苯甲酸 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]