蜂胶提取物制备工艺研究  

Preparation technology of propolis extract

作　　者：吕武清[1] 文萍[1] 姚剑平[1] 范婷婷[1] 胡棠洪 俞建辉 李淑岚 

机构地区：[1]江西省中医药研究院,江西南昌330046 [2]江西省保健品协会,江西南昌330046 [3]江西汪氏蜜蜂园有限公司,江西新建330100

出　　处：《中草药》2014年第6期791-794,共4页Chinese Traditional and Herbal Drugs

基　　金：国家食品药品监督管理局科技计划(食药监许函[2011]212)

摘　　要：目的优化蜂胶提取物制备工艺。方法以浸膏得率,总黄酮、白杨素、乔松素和高良姜素质量分数为指标,采用单因素和正交试验,比较不同提取方法、提取溶剂、提取次数、提取时间、溶剂用量对蜂胶提取物制备工艺的影响。结果蜂胶提取物最佳工艺:蜂胶冷藏后粉碎,加5倍量95%乙醇浸渍(加搅拌)提取4 h,滤过,回收乙醇,浓缩、干燥。结论优化后的蜂胶提取物制备工艺简单、合理,适于进一步推广应用。Objective To establish the preparation technology of propolis extract. Methods With the contents of total flavonoids, chrysin, pinocembrin, and galangin and extract yield as evaluation indexes, the extraction methods, extraction solvent, extraction times, extraction time, and amount of solvent extraction were optimized with single factor test and orthogonal design to investigate the effect on the preparation technology of propolis extract. Results The optimal extraction technology was that propolis was refrigerated, grinded, soaked with 95% five times the amount of ethanol, shaked, then extracted for 4 h, filtrated, concentrated, and dried after ethanol recovery. Conclusion The optimial extracting technology of propolis is stable and feasible, and suitable for further application.

关 键 词：蜂胶提取物 提取工艺 总黄酮 白杨素 乔松素 高良姜素 

分 类 号：R284.2[医药卫生—中药学]