干姜中有效成分在大鼠体内组织分布与归经的相关性研究  

Correlation research between in vivo tissue distribution in rats and channel tropism of active constituents in dried ginger

作　　者：郭敏娟[1] 张廷[1] 马开[2] 崔瑛[1] 汪晶[1] 杨晶晶[1] 王君明[1] 冯志毅[1] 

机构地区：[1]河南中医学院,河南郑州450046 [2]河南省中医药研究院,河南郑州450004

出　　处：《中草药》2014年第7期965-972,共8页Chinese Traditional and Herbal Drugs

基　　金：河南省郑州市科技领军人才项目(121PLJRC534);河南中医学院科技创新团队计划(2011XCXTD01)

摘　　要：目的研究干姜成分6-姜酚、6-姜烯酚、8-姜酚在大鼠体内的组织分布,探讨干姜的归经。方法采用"病证-药效-药动学"方法,给脾胃虚寒模型大鼠ig干姜水提液,分别在10、20、40、60、90、120、360 min时取血、以及心脏、肝脏、脾脏、肺、肾、脑、胃、大肠、小肠等组织,采用高效液相色谱法测定干姜成分6-姜酚、6-姜烯酚、8-姜酚在脾胃虚寒给药组大鼠和正常给药组大鼠体内各组织中的浓度,并计算药动学的相关参数。结果在脾胃虚寒给药组中,3种成分的量以胃、小肠、肝、肺中浓度最高;在正常给药组中,以胃、肾、小肠、大肠、肺中浓度最高。在胃、小肠等消化器官中,脾胃虚寒给药组的浓度明显高于正常给药组。结论干姜主要成分分布以归脾、胃、肺、肝等脏腑为主经,与传统干姜归经理论基本相符。Objective To observe the in vivo tissue distribution of 6-gingerols, 6-shogaol, and 8-gingerols from dried ginger （the rhizome of Zingiber officinale） in rats and to discuss the channel tropism of them. Methods The method of ＂symptoms-efficacy-pharmacokinetics＂ was used and the ginger solution was ig given to the rats which were deficiency-cold in spleen and stomach; Then the blood, heart, liver, spleen, lung, kidney, brain, stomach, large intestine, and small intestine were immediately taken out after the rats were ig given the medince in 10, 20, 40, 60, 80, 120, and 360 min, respectively; Finally, HPLC was used to detect the concentration of 6-gingerols, 6-shogaol, and 8-gingerols from dried ginger in different tissues of rats in each group of deficiency-cold in spleen and stomach and normal by calculating the pharmacokinetic parameters. Results We found that in the group of deficiency-cold in spleen and stomach, the concentration of the three active components was the highest in stomach, small intestine, liver, and lung, and in the group of normal, the three components were mostly distributed in the stomach, kidney, small intestine, large intestine, and lung. What＇s more, in the digestive organs, such as stomach and small intestine, the concentration was obviously higher in the group of deficiency-cold in spleen and stomach than that in the group of normal. Conclusion The main ingredients of dried ginger mostly distribute in spleen, stomach, lung, and liver. This view conform to the traditional channel trpism of dried ginger in traditional Chinese medicine theory.

关 键 词：脾胃虚寒 6-姜酚 8-姜酚 6 姜烯酚 组织分布 归经 

分 类 号：R285.5[医药卫生—中药学]