丹芪偏瘫胶囊对大鼠实验性脑缺血再灌注损伤的保护作用  

Protection of Danqi Piantan Capsule on experimental cerebral ischemia-reperfusion injury model in rats

作　　者：王洋[1] 陈涛[1] 张艳军[2] 李正[1] 崔维利[1] 李进[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津中医药大学中药学院,天津300193

出　　处：《中草药》2014年第7期978-981,共4页Chinese Traditional and Herbal Drugs

基　　金：天津市科技创新专项资金项目(06FZZDSH00408);天津市西青区科技创新专项计划项目(XQCXZX2012-006)

摘　　要：目的采用大鼠脑缺血再灌注模型,研究丹芪偏瘫胶囊对脑缺血的保护作用,并初步探讨其机制。方法180只雄性SD大鼠随机分为假手术组,模型组,阳性药组(银杏叶片6.58 mg/kg),丹芪偏瘫胶囊高、中、低剂量(306、153、76 mg/kg)组。各组大鼠每天ig给药1次,连续给药3 d,于第3次给药后1 h制备左侧大脑中动脉栓塞(MCAO)模型。术后24 h眼内眦取血,测定血清超氧化物歧化酶(SOD)活性、内皮素(ET)水平。术后72 h取脑组织,红四氮唑(TTC)染色法测定大脑梗死面积、脑指数。结果与模型组比较,丹芪偏瘫胶囊高、中剂量组能明显降低大鼠脑缺血再灌注损伤后的脑梗死指数和脑指数(P<0.05),丹芪偏瘫胶囊各剂量组均可增强大鼠血清SOD的活性,其中高、中剂量组与模型组比较差异显著(P<0.05);丹芪偏瘫胶囊各剂量组可降低大鼠血清ET水平,但与模型组比较差异无显著性。结论丹芪偏瘫胶囊对大鼠脑缺血再灌注损伤具有一定的保护作用,其作用机制可能与减轻脑水肿、抗氧化及抑制ET生成有关。Objective To study the protective effect ofDanqi Piantan Capsule （DPC, a capsule made of Salviae Miltiorrhizae Radix, Astragali Radix, Paeoniae Rugra Radix, etc. for the poststroke recovery） on the cerebral ischemia in the rat model of cerebral ischemia-reperfusion and its mechanism. Methods One hundred and eighty male SD rats were randomly divided into six groups： Sham operation, model, positive drug （6.58 mg/kg）, the high-, mid-, and low-dose （306, 153, and 76 mg/kg） DPC groups. The rats were ig administered once daily for consecutive 3 d, and the rat models with left middle cerebral artery occlusion （MCAO） were established at 1 h after the last administration. The blood samples were collected from the fossa orbitalis vein of rats at 24 h after the surgery, used to determine the content of serum superoxide dismutases （SOD） and endothelin （ET）. The brain tissue samples were collected at 72 h after the surgery for determining the cerebral infarct size and cerebral index through TTC-staining. Results Compared with the model group, the high- and mid-dose DPC could significantly reduce （P 〈 0.05） the cerebral index for the cerebral ischemia-reperfusion injury of rats; The three groups of DPJ all could enhance the activity of SOD, especially the high- and mid-dose groups had the significant difference （P 〈 0.05）; The three groups also could reduce the content of ET, but there was no significant difference. Conclusion DPC has the protective effect on cerebral ischemia-reperfusion and its mechanism may be related with relieving cerebral edema, anti-oxidantion, and inhibiting ET.

关 键 词：丹芪偏瘫胶囊 脑缺血再灌注 脑梗死 抗氧化 内皮素 

分 类 号：R285.5[医药卫生—中药学]