复合抗原ESAT-6/CFP-10/Rv0315在活动性肺结核辅助诊断中的价值  

Diagnostic value of multiple antigens ESAT-6 / CFP-10 / Rv0315 in detection of active pulmonary tuberculosis

作　　者：陈翠翠[1] 叶娟[2] 赵俊伟[3] 张舒林[3] 孙战强[4] 汤兵祥[5] 

机构地区：[1]郑州大学第一附属医院呼吸内科,郑州450052 [2]河南中医学院医学免疫学重点实验室,郑州450008 [3]上海交通大学基础医学院病原生物学教研室,上海200025 [4]复旦大学生命科学学院,上海200433 [5]河南省胸科医院呼吸内科,郑州450008

出　　处：《郑州大学学报（医学版）》2014年第2期211-214,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金："十二.五"国家科技重大专项2013ZX10003002-005;国家自然科学基金81271794;上海市科委科技支撑项目12441903300;河南省卫生厅科技攻关项目201203163

摘　　要：目的:评价结核分枝杆菌复合抗原ESAT-6/CFP-10/Rv0315在活动性肺结核诊断中的应用价值。方法:选取初治肺结核病患者70例和健康体检者34例作为结核组和健康对照组,分离PBMCs,分别用复合抗原ESAT-6/CFP-10/Rv0315、ESAT-6肽段库和CFP-10肽段库作为抗原进行ELISPOT实验,检测斑点形成细胞数。结果:复合抗原ESAT-6/CFP-10/Rv0315、ESAT-6肽段库和CFP-10肽段库检测结核分枝杆菌感染的阳性率依次为85.7%(60/70)、77.1%(54/70)、72.9%(51/70),特异性依次为82.4%(28/34)、85.3%(29/34)、88.2%(30/34)。结论:复合抗原ESAT-6/CFP-10/Rv0315作为抗原建立的ELISPOT检测方法在活动性肺结核的辅助诊断中有较高的应用价值。Aim：To evaluate the application value of multiple antigens ESAT-6/CFP-10/Rv0315 in the detection of active pulmonary tuberculosis .Methods：A total of 70 patients with initial pulmonary tuberculosis and 34 healthy persons were enrolled as tuberculosis group and control group .PBMCs isolated from two groups were co-cultured with ESAT-6/CFP-10/Rv0315 , ESAT-6 and CFP-10 antigens .SFCs were enumerated by ELISPOT .Results： The sensitivity of ESAT-6/CFP-10/Rv0315, ESAT6 and CFP-10 antigens by ELISPOT was 85.7%（60/70）, 77.1%（54/70）, and 72.9%（51/70）, respectively.Specificity was 82.4%（28/34）, 85.3%（29/34）, and 88.2%（30/34）, respectively.Conclusion：Multiple antigens ESAT-6/CFP-10/Rv0315 showed certain value for auxiliary diagnosis of active pulmonary tuberculosis .

关 键 词：肺结核 结核分枝杆菌 ESAT-6 CFP-10 Rv0315 

分 类 号：R521[医药卫生—内科学]