种植窗口期人子宫内膜组织中ER、PR及HOXA-11的表达  

Expressions of ER,PR,and HOXA-11 in human endometrium during window period

作　　者：刘子霞[1] 赵华[2] 王兴玲[3] 徐玲[1] 

机构地区：[1]中国人民解放军第91中心医院生殖中心,焦作454150 [2]华中科技大学附属同济医院生殖中心,武汉430032 [3]郑州大学第三附属医院生殖中心,郑州450052

出　　处：《郑州大学学报（医学版）》2014年第2期215-218,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关项目2011020054

摘　　要：目的:探讨雌孕激素受体(ER、PR)及同源框基因-11(HOXA-11)在人子宫内膜种植窗口期的表达。方法:采用免疫组化和RT-PCR方法检测ER、PR及HOXA-11在拟行IVF-ET患者排卵后子宫内膜种植窗口期的表达。按IVF-ET后是否妊娠将153例因输卵管因素不孕的患者分为未妊娠组(n=86)和妊娠组(n=67)。结果:免疫组化结果显示PR和HOXA-11在妊娠组腺上皮细胞中的表达均低于未妊娠组(P均<0.001),在间质细胞中的表达均高于未妊娠组(P均<0.001)。RT-PCR结果显示妊娠组子宫内膜ER、PR及HOXA-11 mRNA的相对表达量均高于未妊娠组(P均<0.05)。P水平与腺上皮、间质PR及腺上皮HOXA-11蛋白表达有关(P均<0.05)。结论:种植窗口期内膜腺上皮PR、HOXA-11表达下调和间质细胞PR、HOXA-11表达上调的失败可能是导致子宫内膜容受性下降的原因之一。Aim：To investigate the expressions of ER , PR and HOXA-11 in human endometrium during window peri-od.Methods：Using immunohistochemical staining and RT-PCR the expressions of ER , PR and HOXA-11 in human endo-metrium during the window period were detected .According to pregnancy or not after IVF-ET, 78 cases of infertility pa-tients with tubal factors were divided into pregnant group （ n=86 ） and non-pregnant group （ n=67 ） .Results： The ex-pressions of PR and HOXA-11 in the glandular epithelium of the pregnant group were significantly lower than those of the non-pregnant group（P〈0.001）, while in the stromal cells were significantly higher than those of the non-pregnant group （P〈0.001）.The expressions of ER,PR and HOXA-11 mRNA of the pregnant group were higher than those of the non-pregnant group（P〈0.05）.There was correlation between level of P and PR,HOXA-11 in glandular epithelial（P〈0.05）. Conclusion：The down-regulated expressions of PR and HOXA-11 in glandular epithelial cells and the up-regulated expres-sions of PR and HOXA-11 in mesenchymal cells may be the reasons which lead to the decrease of endometrial receptivity .

关 键 词：不孕症 子宫内膜容受性 HOXA-11基因 雌激素受体 孕激素受体 

分 类 号：R711.6[医药卫生—妇产科学]