脂肪干细胞复合Ⅰ型胶原对裸鼠移植组织生长的影响  

Effects of adipose tissues derived stem cells compounding with type I collagen on growth of transplanted tissue in nude mice

作　　者：许颖溦 刘林嶓[1] 徐永飞[1] 

机构地区：[1]郑州大学第一附属院整形外科,郑州450052

出　　处：《郑州大学学报（医学版）》2014年第2期255-257,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨脂肪干细胞(ADSCs)复合Ⅰ型胶原在裸鼠背部组织工程化软组织存活与生长的变化。方法:根据所选移植物不同,将实验分为ADSCs复合Ⅰ型胶原(A)、单纯脂肪颗粒(B)和单纯ADSCs(C)组。分别将3组移植物定量注射到每只裸鼠背部皮下,1只裸鼠每种组织复合物各注射一点,并做相应的分组标记,12只裸鼠均做同样处理。观察各移植组织的生长情况,3个月后获取移植组织标本,测量体积、湿重和微血管密度(MVD)。结果:①A组移植组织被吸收较少,体积最大;B组移植组织被吸收较多,体积较小;C组移植组织被吸收最多,体积最小;差异有统计学意义(F=47.619,P<0.001)。②A组移植组织湿重高于B、C组,差异有统计学意义(F=68.425,P<0.001)。③A组移植组织中MVD高于B、C组,差异有统计学意义(F=46.730,P<0.001)。结论:Ⅰ型胶原作为生物支架可促进移植的ADSCs成活与生长,并可加速移植组织血管化。Aim：To observe the effects of compositing adipose tissues derived stem cells （ADSCs） with typeⅠcollagen on nude mice subcutaneous stratum .Methods： Three groups were divided depending on the selected material .Group A （ADSCs companding with type Ⅰcollagen）, group B（pure fat particles）, and group C（pure ADSCs）.The same quantita-tive tissue was injected into one point of subcutaneous stratum of every nude mice , and marked accordingly .The number of nude mice used in the experiment was 12.The growth of the transplanted tissue was observed .The appropriate specimens were obtained three months later and the general observation ,mass（wet weight）, and microvessel density（MVD） were ob-served.Results：The tissue of group A was absorbed least than the other 2 groups, the tissue of group B was absorbed more, the tissue of group C was absorbed most （F=47.619,P〈0.001）.Group A was heavier than group B and C （F=68.425,P〈0.001）.The MVD of group A was higher than group B and C （F=46.730,P〈0.001）.Conclusion： As a cellular scaffold, type Ⅰcollagen can promote the survival and growth of transplanted ADSCs , and can promote neovascu-larization in nude mice subcutaneous stratum .

关 键 词：Ⅰ型胶原 脂肪干细胞 支架 整形外科 组织工程 裸鼠 

分 类 号：R318[医药卫生—生物医学工程]