组织工程骨膜修复兔大段骨缺损的可行性实验研究  

Feasible Study of Tissue Engineered Periosteum Repairing Rabbit Large Segmental Bone Defect

作　　者：拓振合[1] 陈晓飞[2] 赵琳[1] 鲁永婷 任广铁[1] 张苍宇[1] 庾佳佳[1] 孙瑞[1] 

机构地区：[1]兰州大学第二医院骨科/甘肃省骨关节疾病重点实验室,兰州730000 [2]甘肃省中医院放射科,兰州73050 [3]兰州大学第二医院产科,兰州730000

出　　处：《世界科技研究与发展》2014年第2期146-149,190,共5页World Sci-Tech R&D

基　　金：国家自然科学基金(30973064);甘肃省人社厅国家留学归国人员优选资助课题(【2010】343号)资助

摘　　要：目的探索组织工程骨膜体内成骨修复兔大段骨缺损的可行性。方法培养新西兰大白兔骨髓间充质干细胞(BMSCs),以成骨诱导剂诱导成骨分化后,与猪小肠粘膜下层(SIS)复合构建组织工程骨膜。扫描电镜(SEM)观察细胞与材料复合情况。选4月龄新西兰大白兔24只,制备单侧桡骨干4 cm缺损模型。随机选12只植入组织工程骨膜,作为实验组;另12只骨缺损旷置,作为对照组。术后6周后摄x线片观察,切取整段桡骨作为标本行HE及Masson染色观察。结果BMscs诱导14 d后可成骨分化。SEM显示构建的组织工程骨膜上黏附大量种子细胞。x线片观察:实验组骨缺损处有长柱状新生骨形成,并与截骨端骨性融合,密度与正常骨相近;对照组骨缺损处无成骨征象,密度同周围软组织影。组织学观察:实验组骨缺损处有新骨形成,新生骨组织中可见丰富的血管腔及不规则髓腔样结构;对照组骨缺损处仅为纤维结缔组织,无骨组织形成。结论以SIS和BMSCs构建的组织工程骨膜有修复兔大段骨缺损的可行性。组织工程骨膜有进一步深入研究、开发的价值和前景。Objective To explore the feasibility of rabbit large segmental bone defects repaired by using tissue engineered periosteum. Methods Rabbit bone marrow mesenchymal stem cells （BMSCs）were cultured and osteogenetically induced in- to osteoblasts. Porcine small intestine submucosa （SIS） scaffold was produced by decellular and a series mechanical and physiochemical procedures. Then tissue engineered periosteum was constructed by combining osteogenic BMSCs and SIS. A 4cm length of radius with natural periosteum around was osteotomied to create large segmental bone defect model in 24 4- month-old New Zealand white rabbits. Then the animals were randomly divided into 2 equal groups. In experimental group, defect area was occupied by tissue engineered periosteum with a shape of capsular tube which was created by suturing the film circling around the both bone ends,while in control group, the defect was untreated. After Six weeks, the large segmen- tal bone defects were checked by X-ray and histological evaluation. Results BMSCs were differentiated into osteoblasts in 14 days. SEM showed abundant seeding cells adhered on tissue engineered periosteum. In experimental group, new bone tissue was formed and bridged the defect gap, of which could be confirmed by x-ray film and histological staining. There exists a lot of newly formed vessels and medullary cavity among of the new bone tissue, but without lymphocytes infiltration. While in the control group, no any osteogenesis either in X ray, or in histological staining inspecting, and the defect space only re- placed by some connective tissue. Conclusion Tissue engineered periosteum has the feasibility to repair the large diaphysis defect. Tissue engineered periosteum is worthy of the further investigation and exploitation for clinic.

关 键 词：骨髓间充质干细胞 小肠黏膜下层 组织工程骨膜 兔 大段骨缺损 

分 类 号：R681.8[医药卫生—骨科学]