家蚕全基因组启动子预测及基于芯片表达谱的精巢偏向表达启动子顺式元件分析  

Prediction of Silkworm Promoters and Analysis of Promoter Cis-elements for Testis-biased Gene Expression Based on Microarray Expression Profile Data

作　　者：查幸福[1] 殷志明[1] 周春燕[1] 李俊龙[1] 张李颖 向仲怀[1] 夏庆友[1] 

机构地区：[1]家蚕基因组生物学国家重点实验室,农业部蚕桑功能基因组与生物技术重点实验室,西南大学生物技术学院,重庆400715

出　　处：《蚕业科学》2014年第2期216-222,共7页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(No.2012CB114600);国家自然科学基金项目(No.31272502)

摘　　要：启动子是基因的重要组成部分,决定mRNA的转录,从而实现生物的有序发育过程。基于家蚕基因组和全长cDNA序列数据,鉴定了1 341条家蚕启动子序列。序列分析显示,在启动子-500~200 bp区间的GC、AT含量分别出现了1次和2次高峰值。TATA框(TATA-box)、起始子(Inr)、CAAT框(CAAT-box)和GC框(GC-box)等元件分别在256、453、200、334个启动子中被鉴定出,前3种元件集中分布于启动子-500~200 bp区间,第4种元件主要分布在-1 600^-300 bp上游较远处。进一步利用家蚕芯片表达谱数据发现23个启动子为精巢偏向表达基因的启动子。顺式元件分析这些精巢偏向表达启动子中含有6个高频率基序(motif),其中3个motif在精巢偏向表达启动子中的出现频率显著高于对照组,暗示这3个motif在精巢偏向表达基因的转录调控中可能起着重要作用。Promoter is an important component of genes that determines the transcription of mRNA to achieve the or- derly development and differentiation of organisms. In present study, we identified 1 341 promoters in silkworm （ Bom- byx mori） genomic and full-length cDNA sequence databases. Sequence analysis showed that GC content and AT content had one and two peak values in the promoter region from -500 to 200 bp, respectively. Elements of TATA-box, Initiator （ Inr）, CAAT-box and GC-box were present in 256, 453, 200 and 334 promoters, respectively. The first three elements were dominantly located in the region from -500 to 200 bp, and the last element was mostly located at the upstream far from -1 600 to -300 bp. Silkworm-based genomic microarray data showed that 23 promoters are for tes- tis-biased gene expression. Cis-element analysis indicated that these promoters for testis-biased expression had 6 pre-dominant motifs, among which 3 motifs had significantly higher occurrence than the promoters for non-tissue specific expression, suggesting that these 3 motifs play an important role in the regulation of testis-biased gene transcription.

关 键 词：家蚕基因组 启动子 结构特征 精巢偏向表达 顺式元件 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q811.4[农业科学—畜牧兽医]