家蚕长形卵基因(elp)的精细定位及候选基因初步分析  

Fine Mapping of Bombyx mori Ellipsoid Egg Geneelp and Preliminary Analysis of Candidate Genes

作　　者：刘先方[1] 马晓[2] 易晓莉[2] 侯成香[3] 李木旺[1,3] 

机构地区：[1]江苏科技大学蚕业研究所,江苏镇江212018 [2]江苏科技大学生物技术学院,江苏镇江212018 [3]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《蚕业科学》2014年第2期344-347,共4页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(No.31372375);江苏省自然科学基金项目(No.BK20131240);江苏省六大人才高峰项目(No.NY-025)

摘　　要：长形卵为家蚕卵形突变型之一,由位于第18连锁群上的隐性基因elp控制。对家蚕长形卵突变基因进行分子定位,有助于研究该卵形突变的发生机制。在对elp基因初步定位的基础上,用STS分子标记对elp基因进行精细定位并筛选候选基因,构建了elp基因的分子标记遗传连锁图,初步将该基因定位在家蚕基因组nsacf2902的标记T1809与T1826之间,候选区域约550 kb。通过检索KaikoBase和SilkDB数据库,对定位区域内具有EST序列的候选基因进行功能分析,并根据候选基因的CDS和EST序列设计引物进行RT-PCR扩增,在扩增的序列范围内,家蚕长形卵品系167elp与正常卵形品系P50没有差异,需要进一步对侯选基因的全长序列进行克隆和测序分析。The ellipsoid egg is one of the egg shape mutations in silkworm （Bombyx mori）. It is controlled by a recessive gene elp located in the 18th linkage group of silkworm. Molecular mapping of silkworm ellipsoid egg mutation gene is helpful for elucidation of the occurrence mechanism of silkworm egg shape mutations. Based on primary mapping to STS markers of elp gene, we performed fine mapping of the elp gene, screened for candidate genes, constructed a genetic linkage map of molecular markers of elp gene, and primarily mapped the elp gene into a range of about 550 kb between marker T1809 and T1826 in silkworm genome nsacf2902. Through Blast searches in KaikoBase and SilkDB, gene function analysis was conducted to the candidate genes with EST sequences within this region. Based on CDS and EST sequences, primers were designed for RT-PCR amplification. No sequence difference of candidate genes was discovered between silkworm variety 167elp with ellipsoid egg and normal variety P50 within the scope of amplified sequences. The full-length sequence oi candidate genes need to be ~urther cloned and sequenced.

关 键 词：家蚕 长形卵基因 精细定位 STS标记 候选基因 

分 类 号：S881.2[农业科学—特种经济动物饲养]