基于生物反应器探究制药废水中活的但非可培养状态细菌的组成  

Explore Viable but Non-culturable Bacteria in Pharmaceutical Wastewater Based on Bioreactor

作　　者：金夷[1] 周小玲[2] 翟竟余[2] 蒋涛[3] 李裕如[3] 梅荣武[3] 奚雯翼 蔡嫣然[4] 王方园[2] 横田明[2,5] 张立新[6] 丁林贤[1,2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江师范大学地理与环境科学学院,浙江金华321004 [3]浙江省环境保护科学设计研究院,浙江杭州310007 [4]浙江师范大学行知学院,浙江金华321004 [5]日本东京大学分子细胞生物学研究所 [6]中国科学院微生物研究所,北京100101

出　　处：《安徽农业科学》2014年第11期3156-3159,共4页Journal of Anhui Agricultural Sciences

基　　金：浙江省自然科学基金(LY13C010002);浙江省环境保护科学设计研究院(2013F50005)

摘　　要：[目的]探究制药废水中活的但非可培养(viable but non-culturable,VBNC)状态细菌的组成与系统关系。[方法]利用土质滤材构建生物反应器,基于复苏促进因子(resuscitation promoting factor,Rpf)对VBNC细菌的复苏刺激生长作用,采用MPN(most probable number)法,稀释平板法分离其中复苏可培养化的VBNC菌株并解析其16S rRNA基因系统关系。[结果]在MPN培养系中,Rpf对部分细菌有生长刺激作用。制药废水中存在对Rpf敏感、复苏可培养化的VBNC优势菌群;制药废水中对Rpf敏感的VBNC优势细菌主要有革兰氏阳性放线菌Microbacterium、Gordonia和Leucobacter属近缘菌,革兰氏阴性菌Candidimonas、Xanthobacter和Aminobacter属近缘菌,其中菌株ZYM1、ZYM3、ZYZR4和ZYXR1可能属于新种。[结论]研究结果揭示了制药废水中存在VBNC状态细菌,为研究其形成机理、复苏活性化机制以及制药废水的深度处理等提供重要的思路与方法。[Objective] To investigate the composition and phylogenetic relationship of viable but non-culturable bacteria in pharmaceutical wastewater [Method] Applied soil filter to build the bioreactor,then used resuscitation promoting factor （Rpf） which promoted the resuscitation and growth of VBNC bacteria,combined with most probable number （MPN） method and dilution plating to isolate the VBNC bacteria and analyzed 16S rRNA gene phylogenetic relationship.[Result] In MPN culture system,Rpf could promote the resuscitation and growth of some bacteria.There were VBNC advantage floras that sensitive to Rpf in,pharmaceutical wastewater.The major culturable VBNC bacteria in pharmaceutical wastewater belonged to the high GC gram positive actinomycetes including genera Microbacterium,Gordonia and Leucobacter,and negative bacteria including genera Candidimonas,Xanthobacter,Aminobacter.There were 4 strains ZYM1,ZYM3,ZYZR4 and ZYXR1 could be potential novel species.[Conclusion] This research revealed there were VBNC bacteria in pharmaceutical wastewater.These results can provide important ideas and methods for further study on VBNC bacteria of the pharmaceutical wastewater,especially the formation mechanism and recovery mechanism of VBNC bacteria and the advanced degradation process improvement of pharmaceutical wastewater.

关 键 词：制药废水 生物反应器 VBNC菌 Rpf 16S rRNA基因序列系统关系 

分 类 号：S181.3[农业科学—农业基础科学]