苯对小鼠骨髓细胞遗传效应的研究  

Study on genetic toxicity of gaseous benzene to mouse bone marrow cells

作　　者：于光艳[1] 陈强[1] 刘晓梅[1] 赵淑华[1] 孙志伟[1,2] 

机构地区：[1]吉林大学公共卫生学院,长春130021 [2]首都医科大学公共卫生学院

出　　处：《中华劳动卫生职业病杂志》2014年第4期246-250,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的 探讨气态苯吸入染毒小鼠骨髓细胞的遗传毒作用机制,为苯暴露人群的早期生物学标志提供实验依据.方法 将雄性小鼠随机分为4组,即对照组,低、中、高剂量苯染毒组（染毒剂量分别为400、800、1600 mg/m3）,每组6只,进行静式染毒,每天2h.连续染毒15d后将小鼠全部处死,采用生物化学方法测定小鼠骨髓细胞超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、髓过氧化物酶（MPO）活力和丙二醛（MDA）含量;微核试验及单细胞凝胶电泳法测定小鼠骨髓细胞DNA损伤;免疫组织化学法测定MPO基因蛋白的表达情况.结果 各剂量苯组SOD活力（88.67±13.58、73.64±4.50、67.63±5.42） U/mg prot明显低于对照组（119.98±9.42） U/mg prot,差异有统计学意义（P＜0.01）;中、高剂量组SOD活力明显低于低剂量组,差异有统计学意义.中、高剂量组GSH-Px活力（705.07±93.75、674.77±86.80） U/mg prot明显低于对照组（940.25±82.63） U/mg prot,差异有统计学意义（P＜0.01）;高剂量组GSH-Px活力明显低于低剂量组（833.98±130.64） U/mg prot,差异有统计学意义（P＜0.05）.MDA含量（22.42±2.67、22.38±3.02、27.66±2.89） nmol/mg prot随染毒剂量的增加而升高,与对照组（12.35±1.58） nmol/mgprot相比,差异有统计学意义（P＜0.01）;高剂量组MDA含量明显高于中、低剂量组,差异有统计学意义（P＜0.05）.微核试验及单细胞凝胶电泳试验结果显示,各剂量组骨髓细胞微核率（4.67±0.82、5.00±0.89、5.33±1.03）‰明显高于对照组（2.50±0.55）‰,中、高剂量组DNA损伤率（22.08、25.68）％也明显高于对照组（7.00）％,差异有统计学意义（P＜0.01）;高剂量组与低剂量组（11.24）％比较,DNA损伤率有统计学意义（P＜0.05）.MPO活力（16.79±2.16、19.46±2.28、22.53±2.76） U/g prot随着染毒剂量的增加而升高,与对照组（12.89±0.74） U/g prot相比,差异有统计学意义�Objective To investigate the mechanism of genetic toxicity of gaseous benzene to mouse bone marrow cells and to provide an experimental basis for the discovery of early biomarkers among benzeneexposed population.Methods Male mice were randomly divided into control group and three benzene-exposed groups （6 mice per group）.The control group was exposed to ambient air,and the three benzene-exposed groups were exposed to different concentrations of benzene （400,800,and 1 600 mg/m3） for 15 days,2 hours per day,in static inhalation chambers.At the end of the 15-day experimental period,the mice were killed.Bone marrow cells were separated from sacrificed mice,and superoxide dismutase （SOD） activity,glutathione peroxidase （GSH-Px） activity,myeloperoxidase （MPO） activity,and malondialdehyde （MDA） content were determined by biochemical methods.DNA damage was evaluated by micronucleus assay and single cell gel electrophoresis （SCGE）.The expression of MPO protein was determined by immunocytochemistry.Results The SOD activities in different dose groups （88.67 ±13.58,73.64±4.50,and 67.63 ±5.42 U/mg prot） were significantly decreased as compared with the control group （119.98±9.42 U/mg prot） （P〈0.01）.Moreover,the SOD activities in medium-and high-dose groups were significantly lower than that of the low-dose group （P〈0.05）.The GSH-Px activities in medium-and high-dose groups （705.07±93.75 and 674.77±86.80 U/mg prot）were siguificantly decreased as compared with that of the control group （940.25±82.63 U/mg prot） （P〈0.01）,and the high-dose group had a significantly lower GSH-Px activity than the low-dose group （674.77±86.80 U/mg prot vs 833.98±130.64 U/mg prot,P〈0.05）.The MDA content of low-,medium-,and high-dose groups （22.42 ±2.67,22.38 ±3.02,and 27.66±2.89 nmol/mg prot） were significantly higher than that of the control group （12.35±1.58 nmol/mg prot） （P〈0.01）,and MDA content was significantly higher in the high-dose group than in the

关 键 词：苯 超氧化物歧化酶 谷胱甘肽过氧化物酶 髓过氧化物酶 丙二醛 DNA 

分 类 号：R114[医药卫生—卫生毒理学]