电针“肝俞”穴对抑郁型胃溃疡大鼠胃窦黏膜、下丘脑组织P物质和海马5-羟色胺的影响  

Effect of Electroacupuncture Stimulation of “Ganshu”(BL 18) on Locomotor,Gastric Mucosal and Hypothalamic SP Immunoactivity and Hippocampal 5-HT Content in Rats with Depression and Gastric Ulcer

作　　者：邓雪[1] 任路[1] 李静[1] 孙剑端[1] 傅沈康 赵守彰[1] 

机构地区：[1]辽宁中医药大学针灸推拿学院,沈阳110847

出　　处：《针刺研究》2014年第2期124-129,共6页Acupuncture Research

基　　金：辽宁省教育厅项目(No.LT 20100072);沈阳市科技局应用基础研究专项(No.F 13-318-1-67);辽宁省科技攻关项目(No.2009225010-36);辽宁省教育厅科学研究一般项目(No.L 2012339)

摘　　要：目的:探讨电针"肝俞"穴对抑郁型胃溃疡的治疗机制。方法:旷场试验后选择合格大鼠60只,按照随机数字表法均分为空白组、模型组、肝俞组、非经非穴组。建立慢性不可预见性刺激抑郁症合并醋酸烧灼胃溃疡大鼠模型。肝俞组采用电针"肝俞"穴,非经非穴组采用电针脐旁开2.5cm非经穴点,直刺6mm,留针20min,每日1次,连续6d,中间休息1d,共治疗13d。旷场试验检测大鼠活动状况,Guth法计算各组大鼠胃溃疡指数,运用免疫组化法检测下丘脑、胃窦黏膜组织中P物质(SP)的表达,酶联免疫法检测海马组织中5-羟色胺(5-HT)的表达。结果:造模后和实验结束时模型组水平和垂直运动均较空白组明显减少,而治疗后肝俞组水平和垂直运动均较模型组显著增加(P<0.01)。模型组胃溃疡指数、胃黏膜和下丘脑中SP表达水平均较空白组明显升高(P<0.01),肝俞组上述指标均较模型组显著降低(P<0.01)。模型组海马5-HT含量较空白组显著降低,肝俞组海马5-HT含量比模型组明显增加(均P<0.01)。以上各指标,非经非穴组与模型组比较差异均无统计学意义(P>0.05),肝俞组与非经非穴组比较差异均有统计学意义(P<0.01)。结论:电针"肝俞"穴可以同时缓解抑郁状态和胃溃疡损伤程度。这可能是电针对脑肠肽表达影响的结果;电针"肝俞"穴通过对中枢内SP、5-HT表达量的影响而改善抑郁状态。Objective To observe the effect of electroacupuncture （EA） stimulation of ＂Ganshu＂ （BL18） on expression of substance P （SP） in the gastric mucosa and hypothalamus tissues and hippocampal 5-hydroxytryptamine （5-HT）content in rats with depression＋ gastric ulcer, so as to explore its mechanism underlying improvement of depressive gastric ulcer. Methods A total of 60 SD rats were equally randomized into normal, model, EA-BL18 and non-acupoint groups. The depression4- gastric ul- cer model was established by chronic unpredictable mild stress （OUMS） paradigm for 21 days in accordance with Willner and col- leagues＇ methods （1987）, and injection of 90% glacial acetic acid （0.01 mL） into the sub-serous layer near the gastric antrum under anesthesia on day 10 after starting OUMS. Following modeling, EA（4 Hz/15 Hz, 2 V） was applied to bilateral ＂Ganshu＂ （BL 18） for 20 min, once daily for 2 weeks, with one day＇s interval between two weeks. The non-acupoint was located about 2.5 cm lateral to the umbilicus and stimulated by EA similar to EA-BL18. The rats＇ locomotor ability was assessed by open field tests （crossing and rearing numbers）. The gastric ulcer index was calculated according to Guth＇s method, and hypothalamic and gastric antrum SP immunoactivity was determined by immunohistochemistry and hippocampal 5-HT content was detected by ELISA. Results After modeling, compared to the normal group, both crossing number and rearing number of open field tests on day 21 and 34 were significantly decreased in the model group （P^0.01）, while compared to the model group, both crossing and rea- ring numbers on day 34 were considerably increased in the EA-BL18 group （P〈0.01） rather than in the non-acupoint group（P〈0.05）. Following modeling, the gastric ulcer index, SP expression levels in the gastric antrum and hypothalamus tissue were obviously increased in the model group （P〈0.01）, and notably decreased in the EA-BL18 group （P〈0.01 ） but not in th

关 键 词：电针 抑郁症 胃溃疡P物质 5-羟色胺 胃黏膜 下丘脑 海马 

分 类 号：R245.97[医药卫生—针灸推拿学]