电针“命门”穴对绝经后骨质疏松症大鼠骨形成蛋白-2的影响  

Effect of Electroacupuncture of “Mingmen”(GV 4) on Bone Morphogenetic Protein-2 Expression in Osteoporosis Rats

作　　者：黄桂榕[1,2] 李沛[3] 林莺[3] 范怀玲[4] 张木兰[4] 秦玮[4] 周棋[4] 纪峰[4] 

机构地区：[1]福建中医药大学附属康复医院,福州350003 [2]国家中医药管理局三级实验室:中医康复技术实验室,福州350003 [3]福建中医药大学海外教育学院,福州350003 [4]福建中医药大学针灸学院,福州350122

出　　处：《针刺研究》2014年第2期130-135,共6页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81173347/H 2718);国家自然科学基金青年科学基金项目(No.81102651/H 2718)

摘　　要：目的:观察电针"命门"穴对绝经后骨质疏松症大鼠股骨骨形成蛋白-2(bone morphogenetic protein-2,BMP-2)及骨生物力学的影响,探讨电针治疗绝经后骨质疏松症的机制。方法:将SD雌性大鼠随机分为假手术组、模型组、命门组、非经非穴组及雌激素组,每组10只。除假手术组外,各组大鼠均切除双侧卵巢制备绝经后骨质疏松症模型。造模成功后,命门组和非经非穴组每日电针"命门"及非经非穴1次,疏密波,频率为2Hz/15Hz,强度为1mA,每次持续20min,10d为1个疗程,治疗3个疗程;雌激素组用戊酸雌二醇片生理盐水混悬液(25μg/mL,2mL/500g)灌胃,连续灌服1个月。治疗后采用免疫组织化学方法检测大鼠左侧股骨BMP-2的表达变化,运用三点弯曲实验检测大鼠右侧股骨最大载荷及断裂载荷。结果:造模后大鼠阴道脱落细胞涂片可见细胞核大,核质比例大,提示去势成功。造模后大鼠股骨骨小梁较假手术组明显变少,结构松散,骨髓腔明显变大,存在大量脂肪和空泡,表现出典型的骨质疏松症组织形态。模型组最大载荷和断裂载荷较假手术组明显降低(P<0.05),命门组及雌激素组最大载荷和断裂载荷较模型组及非经非穴组明显升高(P<0.05)。模型组BMP-2表达较假手术组明显减弱(P<0.05),命门组及雌激素组BMP-2表达较模型组明显增强(P<0.05)。结论:电针"命门"穴能增加BMP-2的表达水平,提高骨生物力学性能,增强骨强度,从而治疗绝经后骨质疏松症。Objective To observe the effect of electroacupuncture （EA） stimulation of ＂Mingmen＂ （GV 4） on the bone morphegenetic protein-2（BMP-2） content and biomechanics in osteoporosis rats so as to explore its mechanism underlying im- provement of osteoporosis. Methods Fifty female SD rats were randomized into sham operation （sham）, model, EA-GV 4, EA-non-acupoint （non-acupoint） and estrogen （medication） groups, with 10 rats in each group. Postmenepausal osteeporosis model was established by removing the rats＇ bilateral ovaries under anesthesia. EA （2 Hz/15 Hz, 1.0 mA） was applied to ＂Ming- men＂ （GV 4） or non-acupoint for 20 min, once daily for 30 times , with one day＇s interval between every two 10 times. Rats of the medication group were lavaged with Pentanoic Acid Estradiel （25 μg/mL, 2 mL/500 g）, once every day （the dosage of estra- diol was adjusted according to their body weight） continuously for 1 month. Rats of the model and sham groups experienced the fixing and fastening procedures as the other rats in the EA and medication groups. After intervention, the BMP-2 expression level of the femoral bone tissue, and bone biomechanical values were determined by immunohistochemistry and three-point bending tests, respectively. Results ① In comparison with the sham operation group, the femoral biochemical maximum load and frac-ture load values were significantly decreased in the model group （P〈0.05）. While compared with the model group, the biochem- ical maximum load and fracture load values were obviously increased in the EA-GV 4 and medication groups（ P〈0.05）, but not in the non-acupoint group （P〈0.05）. ② Compared with the sham group, the femoral BMP-2 expression of model group was signifi- cantly decreased （P〈0.05）,compared with the model group, the expression of BMP-2 of GV 4 and medication groups signifi- cantly increased （P^0.05）. Cenclusien EA-GV 4 intervention can improve bone biomechanical changes in osteoporosis rats.

关 键 词：绝经后骨质疏松症 电针干预 命门穴 股骨生物力学 骨形成蛋白-2 

分 类 号：R245.9[医药卫生—针灸推拿学]