定量PCR比较两种苦瓜制品对人肠道菌群结构的体外调节作用  

In vitro structural modulation of human gut microbiota by two bitter melon preparations: a comparison using qPCR

作　　者：何欣欣[1] 徐佳[1] 宋岷埈 张旭[1] 龙文敏[1] 王凌华[1] 

机构地区：[1]上海交通大学生命科学技术学院微生物代谢国家重点实验室,上海200240

出　　处：《中国微生态学杂志》2014年第4期382-387,共6页Chinese Journal of Microecology

基　　金：国家自然基金创新群体(31121064)

摘　　要：目的通过体外厌氧培养联合定量PCR的方法比较两种不同的苦瓜制品,苦瓜全粉(Bitter melon power,BMP)和苦瓜乙醇提取物(Bitter melon ethanol extract,BME),对健康人和2型糖尿患者肠道菌群结构的调节作用。方法采集一名健康个体和一名2型糖尿病个体的新鲜粪便样品,在体外厌氧的条件下与不同浓度的BMP或BME共培养,收集0、12、24、48 h的菌体沉淀。采用定量PCR的方法,检测乳杆菌属细菌、双歧杆菌属细菌、丁酸盐产生菌、硫酸盐还原菌和肠杆菌科细菌这5类细菌在总菌中相对含量的变化。结果培养过程中,双歧杆菌属细菌、硫酸盐还原菌和肠杆菌科细菌在两名个体培养样本中表现出类似的生长趋势,而乳杆菌属细菌和丁酸盐产生菌则有差异。相同培养时间下,与对照组相比,BMP和BME在两名个体的培养样本中均能富集乳杆菌属细菌、双歧杆菌属细菌和丁酸盐产生菌,并抑制肠杆菌科细菌,但是只有BMP 15 mg/mL能持续抑制硫酸盐还原菌。相同浓度之下,BMP对菌群结构的调节效果比BME显著。结论来自不同个体的相同肠道细菌类群可能呈现不同的体外生长趋势,但BMP和BME均能对同一细菌类群起到类似的调节作用;且相较于BME,BMP通过富集一些有益细菌并抑制机会致病细菌从而改善肠道菌群结构的效果更显著。Objective To evaluate the effects of two different bitter melon preparations （Bitter melon power, BMP vs Bitter melon ethanol extract, BME） with regard to their structural modulation of gut microbiota in a healthy individual and a T2D patient in vitro. Methods Fresh fecal samples from a healthy individual and a T2D patient were co-cultivated with BMP or BME in different concentrations under anaerobic conditions. Quantitative PCR （qPCR） was employed to estimate the relative abundance of Lactobacillus, Bifidobacterium, butyrate-producing bacteria （BPB） , sulphate-reducing bacteria （SRB） and Enterobacteriaceae in total bacteria at 0, 12, 24 and 48 h. Results During the cultivation, similar growth profile was observed in Bifidobacterium, SRB and Enterobacteriaceae but not in Lactobacillus or BPB in both the two individuals. Compared with the control, both BMP and BME could enrich Lactobacillus, Bifidobacterium and BPB during cultivation, while Enterobacteriaceae was significantly inhibited. However, only BMP （ 15 mg/mL） prevented the increase of SRB continuously. Conclusion The same bacterial group from different individuals might have different growth profile, but could be modulated by BMP or BME in a similar way. BMP may have a more significant role in improving the gut microbiota structure by enriching specific beneficial bacteria and inhibiting potential pathogens than BME.

关 键 词：苦瓜全粉 苦瓜乙醇提取物 肠道菌群 定量PCR 

分 类 号：R378[医药卫生—病原生物学]