应用 SRAP 分子标记构建黄麻遗传资源 DNA 指纹图谱  

The establishment of DNA fingerprintings of jute germplasm resources with SRAP molecular markers

作　　者：陈惠端[1] 陈美霞[2] 蔡金月 徐鲜钧 方平平[5,6] 徐建堂[5,6] 陶爱芬[5,6] 牛小平[5,6] 祁建民[5,6] 

机构地区：[1]福建农林大学机电工程学院,福建福州350002 [2]宁德师范学院生物系,福建宁德352100 [3]漳浦县种子管理站,福建漳浦363200 [4]武夷山生物研究所,福建武夷山354300 [5]福建农林大学作物遗传育种与综合利用教育部重点实验室 [6]福建农林大学农业部东南黄红麻科学观测实验站,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2014年第2期113-118,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30571188);国家麻类产业技术体系建设项目(nycytx-19-E05);农业部东南黄红麻科学观测实验站项目(农科教2011-9)

摘　　要：以36个黄麻野生种和栽培种为材料,对其基因组DNA进行相关序列扩增多态性(SRAP)分子标记分析.结果表明:从50对引物中筛选出扩增带型好、品种间带型差异明显、易于识别的34对多态性引物,共扩增出1845条多态性条带,平均每对引物扩增出51.25条,最后从中筛选出14对核心引物构建了36个黄麻品种的DNA指纹图谱,每个品种均有各自特异的DNA指纹,可为黄麻种质资源分子身份证绘制提供依据.Jute is an important fiber crop in textile industry and paper manufacture. The objective of this study is to establish se- quence related amplified polymorphism （SBAP） finserpfints of 36 representative jute varieties at home and abroad. A total of 50 SRAP was used to survey the polymorphism, the result showed 34 pairs of SRAP primers could amplify polymorphism bands and revealed 1845 polymorphic loci in total, with an average of 51.25 polymorphic bands per primer pair. Then 14 pairs core primers were selected. They could distinguish the 36 jute varieties to establish the DNA fingerprints, in which each variety had its unique fingerprinting pattern.

关 键 词：黄麻 种质资源 相关序列扩增多态性(SRAP) DNA指纹图谱 

分 类 号：S563.4[农业科学—作物学]