低温快速成型亲水改性复合人工骨支架的性能测定  

Preparation and properties of hydrophilic modified artificial bone scaffold using low-temperature rapid prototyping

作　　者：何美健[1,2] 王大平[1,2,3] 黄江鸿[1,2,3] 张明[4] 

机构地区：[1]安徽医科大学深圳二院临床学院,广东省深圳市518035 [2]深圳市第二人民医院骨科,广东省深圳市518035 [3]深圳市组织工程重点实验室,广东省深圳市518035 [4]中科院深圳先进技术研究院,广东省深圳市518035

出　　处：《中国组织工程研究》2014年第12期1824-1830,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然科学基金(S2012010008129);深圳市科技资金研发项目(CXZZ2012061416023484);深圳市重点实验室提升项目(CXB201104220049A)~~

摘　　要：背景:低温快速成型技术具有支架成型可控性、保持材料生物学活性和易于实现支架材料的三维多孔立体结构等优势,被迅速用于骨组织工程支架的制备。目的:采用低温快速成型制备聚乙二醇改性聚乳酸-乙醇酸/纳米羟基磷灰石复合支架,并检测其性能。方法:采用低温快速成型设备分别制备聚乙二醇改性聚乳酸-乙醇酸/纳米羟基磷灰石与聚乳酸-乙醇酸/纳米羟基磷灰石复合支架,通过电镜观察支架超微结构,以介质(乙醇)浸泡法测定支架孔隙率,采用电子试验机检测支架力学性能;将两种支架材料分别与大鼠成骨细胞共培养,培养12 h采用沉淀法检测细胞黏附率,培养1,3,5,7,9,12 d采用CCK-8法检测细胞增殖。结果与结论:两组支架孔径均在理想范围内并具有较高孔隙率,但聚乙二醇改性聚乳酸-乙醇酸/纳米羟基磷灰石支架的孔径波动范围大,孔径均值较聚乳酸-乙醇酸/纳米羟基磷灰石支架小且部分有闭塞现象。聚乙二醇改性聚乳酸-乙醇酸/纳米羟基磷灰石支架的细胞黏附率及表面细胞增殖活性高于聚乳酸-乙醇酸/纳米羟基磷灰石支架(P<0.05),力学性能低于聚乳酸-乙醇酸/纳米羟基磷灰石支架(P<0.05)。表明聚乙二醇改性聚乳酸-乙醇酸/纳米羟基磷灰石复合支架具有良好的细胞相容性。BACKGROUND：Low-temperature rapid prototyping technology is a new kind of rapid prototyping technology, and it is rapidly used in the preparation of bone tissue engineering scaffolds because it can make scaffold forming control able and can keep the biological activity of the materials, also can easily realize the scaffold with porous of three-dimensional structure and other advantages. OBJECTIVE：To investigate the preparation process of polyethylene glycol-modified polylactic acid-glycolic acid/nano-hydroxyapatite （PLGA-PEG/n-HA） using the low-temperature rapid prototyping, and to test its performance. METHODS：PLGA-PEG/n-HA and PLGA/n-HA were prepared by low-temperature rapid prototyping equipment. Under an electron microscopy, we observed ultra-structure of the scaffolds. Immersion （ethanol） method was used to test the porosity, and electronic testing machine was used to determine the material mechanical properties. Then these two kinds of scaffolds with rat osteoblasts were cultured in vitro, the cel adhesion rate was detected by precipitation method after 12 hours, and cel counting kit-8 method was used to determine the cel＆amp;nbsp;proliferation at culture days 1, 3, 5, 7, 9, 12. RESULTS AND CONCLUSION：Both of the two scaffolds had ideal aperture range and high porosity. But the aperture range of PLGA-PEG/n-HA scaffolds had large fluctuations, and the average aperture was smal er than that of PLGA/n-HA. Some pores were closed up. The cel adhesion rate and the cel growth curve of PLGA-PEG/n-HA was better than that of PLGA/n-HA （P＜0.05）, but the mechanical properties were less than PLGA/n-HA （P＜0.05）. The results showed the PLGA-PEG/n-HA scaffolds had good cel compatibility.

关 键 词：生物材料 骨生物材料 低温快速成型 聚乳酸-乙醇酸 聚乙二醇 纳米羟基磷灰石 骨组织工程 复合支架 骨缺损 亲水改性 广东省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]