纳米含氟羟基磷灰石牙种植体的生物相容性  

Biocompatibility of nano fluoridated hydroxyapatite for dental implants

作　　者：游滢滢[1] 冯云枝[2] 

机构地区：[1]中山大学附属第五医院口腔科,广东省珠海市519000 [2]中南大学湘雅二医院口腔医疗中心,湖南省长沙市410012

出　　处：《中国组织工程研究》2014年第12期1901-1906,共6页Chinese Journal of Tissue Engineering Research

基　　金：中南大学米塔尔创新创业项目重点资助(07MX30)~~

摘　　要：背景:有关纳米含氟羟基磷灰石牙种植体材料生物相容性的报道较少。目的:检测纳米含氟羟基磷灰石牙种植体材料的体外生物相容性。方法:采用溶胶凝胶技术分别制备羟基磷灰石与纳米含氟羟基磷灰石。①溶血性实验:在0.2 mL稀释兔抗凝血中分别加入0.01,0.15,0.2 g/L纳米含氟羟基磷灰石溶液、生理盐水及蒸馏水各10 mL,检测各组上清液吸光度值。②体外细胞毒性实验:分别以100%,50%纳米含氟羟基磷灰石浸提液、100%羟基磷灰石浸提液、苯酚溶液及RPMI1640培养液培养传至第2代的L929细胞,MTT法检测培养2,4,7 d的吸光度值。结果与结论:体外溶血性实验显示,各浓度梯度纳米含氟羟基磷灰石的溶血率均在5%以内,符合医用材料的溶血要求。体外细胞毒性实验显示,随着培养时间的增加,100%,50%纳米含氟羟基磷灰石浸提液组细胞贴壁覆盖率增加,细胞密度增高,细胞为长梭形或多角形,细胞增殖及形态与RPMI1640培养液组、羟基磷灰石组无明显差别,细胞毒性为0级。BACKGROUND：Few reports focus on biocompatibility of nano fluoridated hydroxyapatite for dental implants. OBJECTIVE：To investigate the biocompatibility in vitro of nano fluoridated hydroxyapatite modified for dental implants. METHODS：We utilized sol-gel method to prepare nano fluoridated hydroxyapatite and nanohydroxyapatite powders. （1） Hemolysis test：0.01, 0.15, 0.2 g/L nano fluorinated hydroxyapatite solution, saline and distil ed water at a volume of 10 mL were added into 0.2 mL diluted rabbit anti-coagulation blood samples, respectively. Then the supernatant was detected by absorbance values. （2） In vitro cytotoxicity test：Passage 2 L929 cel s were respectively cultured in culture media containing 100%, 50%nano fluorinated hydroxyapatite extract, 100%hydroxyapatite extract, phenol solution and RPMI1640. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay was employed to measure absorbance values at days 2, 4, 7 of culture. RESULTS AND CONCLUSION：Hemolysis test in vitro showed the hemolysis rates of nano fluorinated hydroxyapatite groups were less than 5%, which were accorded with haemolysis demand of medical materials. The cytotoxicity test in vitro showed during cultivation, the adherence rate of L929 cel s cultured in 100%and 50%nano fluorinated hydroxyapatite extracts were increasing and cel density was rising up. Cel s were fusiform or polygon, which had no evident differences from negative controls in morphology. Nano fluorinated hydroxyapatite showed nontoxic to L929 cel s in vitro.

关 键 词：生物材料 口腔生物材料 羟基磷灰石 含氟羟基磷灰石 种植体 生物相容性 纳米材料 

分 类 号：R318[医药卫生—生物医学工程]