自组装多肽纳米纤维水凝胶在细胞三维培养中的应用及特征  

Application and characteristics of self-assembling peptide nanofiber hydrogel in three-dimensional cell culture systems

作　　者：刘茜[1,2] 王秀梅[2] 

机构地区：[1]苏州大学现代丝绸国家工程实验室,纺织与服装工程学院,江苏省苏州市215123 [2]清华大学材料科学与工程学院,再生医学与仿生材料研究所,北京市100084

出　　处：《中国组织工程研究》2014年第12期1907-1913,共7页Chinese Journal of Tissue Engineering Research

基　　金：江苏省高校自然科学研究面上项目(13KJB430019);中国博士后科学基金(2013M541724);清华大学自主科研计划(20121087982)~~

摘　　要：背景:自组装多肽类材料因其独特的设计及良好的生物相容性和可降解性在众多三维支架材料中脱颖而出。目的:综述RADA类离子互补型自组装多肽支架材料的结构和功能化设计,从细胞三维培养方面探讨多肽类材料作为细胞载体材料在细胞治疗中的应用前景。方法:由作者通过PubMed、Web of science数据库及CNKI数据库检索有关自组装多肽水凝胶的相关文献,检索词为"self-assembly peptide,tissue engineering;自组装多肽,组织工程",检索文献量总计224篇,纳入包含多肽材料设计、功能化多肽材料、多肽材料用于细胞三维培养方面的研究,最终纳入48篇。结果与结论:从物理结构角度讲,多肽材料可以在生理环境中自组装成具有纳米级纤维和较高孔隙率的水凝胶,最大程度上模拟细胞外基质的结构,保障细胞生存在一个真正的三维环境中。从生物功能角度讲,多肽材料可以根据不同需求复合特异性的生物活性短肽片断,赋予材料一定的细胞特异性,可以促进细胞的黏附、增殖或分化。BACKGROUND：Self-assembling peptide scaffolds have attracted much more attention among three dimensional biomaterials due to its biocompatibility, biodegradation, and tailor-made properties. OBJECTIVE：To review the structure and design of functionalized self-assembling RADA peptide, and the recent advances in the use of RADA self-assembling peptide for three-dimensional cel culture in cel therapy applications. METHODS：Total 224 literatures related to self-assembling peptide and tissue engineering from PubMed, Web of Science and CNKI databases were screened out for this review. The keywords were“self-assembly peptide, tissue engineering”in English and Chinese, respectively. Final y 48 of 224 articles about the design, fictionalization, and three-dimensional cel culture of peptide scaffolds were included in this review. RESULTS AND CONCLUSION：Self-assembling peptide could undergo spontaneous assembly into wel-ordered interwoven nanofibers in water and rapidly form hydrogel, which physical y mimics the architecture of natural extracel ular matrix to ensure a real three-dimensional microenvironment for cel s. In terms of bio-function, this material can be tailor-made with various bioactive short peptide motifs to promote cel adhesion, proliferation, and differentiation.

关 键 词：生物材料 纳米生物材料 自组装多肽 水凝胶 纳米纤维 三维培养 生物材料综述 

分 类 号：R318[医药卫生—生物医学工程]