Toll样受体4多态性调节白细胞介素1-α表达与宫颈癌相关性  

IL-1α expression modified by the Toll-like receptor 4 polymorphisms is associated with cervical carcinoma

作　　者：戴穹[1] 张旭[2] 董晖[2] 杨志惠[2] 

机构地区：[1]泸州医学院解剖学教研室,泸州646000 [2]泸州医学院病理学教研室,泸州646000

出　　处：《解剖学杂志》2014年第2期166-169,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（81101679）

摘　　要：目的：研究Toll样受体4（TLR4）多态性与宫颈癌相关性及可能的机制.方法：246名宫颈癌患者和年龄相匹配的246名健康对照个体TLR4 A896G、D299G和T399I多态性位点基因型用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测;ELISA方法检测宫颈癌组织匀浆中白细胞介素1-α（IL-1α）、白细胞介素-8（IL-8）、转化生长因子-β（TGF-β）和肿瘤坏死因子-α（TNF-α）蛋白水平.结果：与T399I CC基因型相比,CT合并TT基因型显著增加宫颈癌的患病风险,而携带T399IT等位基因的个体患病风险是C等位基因个体的1.912倍,TLR4 A896G和D299G多态性基因型在病例-对照组中频率无差异;IL-1α浓度在T399I CT合并TT基因型个体宫颈癌组织中的表达高于T399I CC组个体.结论：TLR4T399I CT合并TT基因型可增加个体患宫颈癌的风险,其机制可能是调节宫颈癌组织中IL-1α蛋白表达.Objective： To investigate the association of TLR4 D299G, T399I and A896G polymorphisms and cervical carcinoma, and to explore the effects of polymorphisms on cytokine in the cervical carcinoma biopsies. Methods： The genotypes of these three loci among 246 patients with cervical carcinoma and 246 healthy controls were determined by polymerase chain reaction-restriction fragment lengthy polymorphism （PCR-RFLP）. Cytokines in cervical carcinoma biopsies were measured by enzyme-linked immunosorbent assay （ELISA）. Results： It showed that CT combined TT genotype of T399I was associated with increased cervical carcinoma risk at OR= 1. 985 （95 %CI： 1. 286-3. 135,P〈0. 01）, individuals with T allele of T399I showed a 1. 912-fold increase in cervical carcinoma risk as compared with the T399I C allele （95%CI： 1. 315- 2. 817, P〈0.01）. Protein levels of IL-la was significantly elevated in patients with T399I CT and TT genotypes. Conclusion： Our data suggest that CT combined TT genotype of TLR4 T399I is associated with increasing cervical carcinoma risk, and the possible mechanism may be modified IL-1α protein expression in the biopsies of cervical carcinoma.

关 键 词：TOLL样受体4 宫颈癌 细胞因子 多态性 

分 类 号：R589.2[医药卫生—内分泌]