不同冷冻方法对人卵裂期胚胎发育潜能的影响  

Effect of different freezing methods on human cleavage-stage embryos development potential

作　　者：阮井玲[1] 王羽[1] 杨健之[1] 陈智勤[1] 潘家坪[1] 梁珊珊[1] 胡烨[1] 韩毅冰[1] 李昆明[1] 滕晓明[1] 

机构地区：[1]同济大学附属第一妇婴保健院生殖医学中心,上海200040

出　　处：《解剖学杂志》2014年第2期175-177,191,共4页Chinese Journal of Anatomy

摘　　要：目的：比较玻璃化法与慢速程序法对人卵裂期胚胎冷冻复苏的效果.方法：将接受体外受精-胚胎移植患者的取卵周期移植后剩余的胚胎,分别进行玻璃化法或慢速程序法冷冻,比较两组胚胎解冻后的复苏率、胚胎完整率、胚胎种植率、临床妊娠率及观察非当日移植的复苏胚胎的发育潜能等指标.结果：玻璃化冷冻组的活产率均显著高于慢速程序化冷冻;玻璃化冷冻后的胚胎复苏率和胚胎完整率显著高于慢速程序化冷冻;复苏周期移植取消率显著低于慢速程序化冷冻;而临床妊娠率和种植率的差异无统计学意义.结论：玻璃化冷冻和慢速程序化冷冻相比有更高的复苏率和胚胎利用率.Objective： To compare the vitrification and slow-freezing on human embryos in cleavage. Methods： After IVF- embryo transfered in oocyte retrieval cycles, the remaining embryos were divided into a vitrification freezing group and a slow- freezing group. The embryo survival rate, implantation rate, clinical pregnancy rate and other indicators were compared between two methods. Results ~ The live births rates of the vitrification group were significantly higher than those of the slow- freezing group; The embryo survival rates and complete embryo rate of the vitrification group was significantly higher than those of the slow-freezing group, and the embryo transfer cancellation rate was significantly lower than that of the slow- freezing group, while the clinical pregnancy rate and implantation rate was slightly higher than those of the slow-freezing group, but the difference was not statistically significant. The developmental potential of the embryos of the vitrification group was significantly better than that of the slow-freezing group. Conclusion.. The vitrification method had a higher survival rate and lower transfer cancellation rate than did the slow-freezing method. Although the implantation rate between the two groups had no significant difference, the vitrification method can preserve the developmental potential of embryos better than slow- freezing method can. These data provide further evidence that vitrification imparts less trauma to cells and is, therefore, a more effective means of cryopreserving the human embryo than conventional slow-freezing is.

关 键 词：玻璃化冷冻 慢速程序化冷冻 冷冻胚胎 卵裂期 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]