hsa-miR-1908上游启动子区的生物信息学分析  

Bioinformatic analysis of the hsa-miR-1908 upstream promoter region

作　　者：匡仟卉柠 李景云[2] 季晨博[2,3] 郭锡镕[2,3] 倪毓辉[3] 徐美玉[1] 

机构地区：[1]南通大学附属医院儿科,江苏南通226001 [2]南京医科大学附属南京妇幼保健院医学研究中心,江苏南京210011 [3]南京医科大学儿科研究所,江苏南京210029

出　　处：《临床儿科杂志》2014年第4期379-383,共5页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.81100618)

摘　　要：目的利用各种生物信息学工具预测hsa-miR-1908上游启动子功能，以进一步研究其在人脂肪细胞中的转录调控机制。方法应用Ensemble数据库获取hsa-miR-1908上游启动子序列，利用多种在线相关软件预测出甲基化部位和转录因子结合部位。结果获取hsa-miR-1908上游启动子序列全长1458bp。miR-1908启动子序列中CpG岛位于（438～756）bp、（836937）bp、（979～1374）bp处，CpG岛的存在会抑制miR-1908启动子的转录。miR-1908有15个转录因子的结合位点。结论miRNA启动子相关生物信息学的研究，提高对启动子的研究效率，为进一步研究miR-1908的转录调控机制提供了重要的信息。Objective To predict the functions of hsa-miR-1908 promoter using various bioinformatic tools, and to provide clues for further study on transcriptional regulation mechanism of miR-1908 in human adipocytes. Methods The promoter se- quence of miR-1908 was obtained from Ensemble, and then the CpG islands and transcription factor binding sites were pre- dicted by a variety of online bioinformatic tools. Results The length of the miR-1908 promoter sequence was 1 458 bp. The CpG islands, which inhibited the transcription of miR-1908, were located at （438-756） bp, （836-937） bp and （979-1374） bp. Meanwhile, 15 transcription factor binding sites were found in the promoter sequence of miR-1908. Conclusions miRNA up- stream promoter related bioinformatics can not only improve the efficiency of microRNA promoter research, but also provide further important information on transcriptional regulation of miR-1908.

关 键 词：miR-1908启动子区 生物信息学分析 转录因子结合部位 

分 类 号：R589.2[医药卫生—内分泌]