adiponectin对脂肪组织创面愈合作用的体外实验研究  

Experimental study in vitro of the effect of adipose tissue wound healing by adiponectin

作　　者：占小波 肖亮[2,3] 葛子豪 周汉新[1,2] 

机构地区：[1]广州医科大学,广东广州518082 [2]深圳市第二人民医院肝胆外科,广东深圳518035 [3]深圳市第二人民医院肿瘤生物治疗科,广东深圳518035 [4]汕头大学医学院第一附属医院普外科,广东汕头515041

出　　处：《中国现代普通外科进展》2014年第3期173-178,共6页Chinese Journal of Current Advances in General Surgery

基　　金：深圳市第二人民医院育苗计划;深圳市科技计划项目(医药卫生类)(201202056);中国博士后科学基金(2013M531895);深圳市知识创新计划资助项目(JCYJ20130401114114947;JCYJ20130401114225775)

摘　　要：目的:探讨脂肪组织创面愈合(ATWH)的发生机制。方法:体外培养小鼠3T3-L1前脂肪细胞系,应用免疫组化及RT-PCR法检测3T3-L1前脂肪细胞脂联素受体(AdipoR1/2)的表达,用Elisa法测定3T3-L1前脂肪细胞自身分泌脂联素(adiponectin)的浓度,应用MTT和体外伤口愈合模型研究不同浓度adiponectin对前脂肪细胞增殖和迁徙的作用。结果:AdipoR1/2可在3T3-L1前脂肪细胞中表达,不同密度的3T3-L1前脂肪细胞分泌的adiponectin浓度差异无统计学意义(P>0.05),adiponectin能够促进3T3-L1前脂肪细胞的增殖和迁徙。结论:adiponectin可能通过促进前脂肪细胞的增殖和迁徙促进ATWH,全身或局部应用adiponectin可能是促进外科切口愈合的有效治疗方法。Objective： To investigate the mechanism of adipose tissue wound healing（ATWH）. Methods： 3T3-L1 preadipocytes were cultured and expression of adiponectin receptors （AdipoR1/2） in 3T3-L1 preadipocytes was detected by immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR）. The concentrations of adiponectin secreted by 3T3-L1 preadipocytes were measured by ELISA. Proliferation and migration of 3T3-L1 preadipocytes were performed in vitro by MiT and wound closure assay, respectively. Results： AdipoR1/2 were ex- pressed in 3T3-L1 preadipocytes. There were no statistically significant differences of adiponectin secreted by 3T3-L1 preadipocytes of different densities （P〉0.05）. Adiponectin can promote 3T3-L1 preadipocytes proliferation and migration. Conclusions： Adiponectin might regulate ATWH by promoting preadipocytes proliferation and migration, and application of adiponectin systemically and/or locally is proposed to be a hopeful therapeutic approach for coping with surgical incision.

关 键 词：创面愈合 脂联素 脂肪组织 前脂肪细胞 增殖 迁徙 

分 类 号：R656[医药卫生—外科学]