人类白细胞抗原HLA-C＊04：01：01G组的区分鉴别  

Discrimination of Alleles in HLA-C ＊ 04：01：01G Groups

作　　者：王炜[1] 陈男英[1] 章伟[1] 何俊俊[1] 韩浙东[1] 秦斐[1] 董丽娜[1] 朱发明[1] 吕杭军[1] 

机构地区：[1]浙江省血液中心、卫生部血液安全研究重点实验室,浙江杭州310006

出　　处：《中国实验血液学杂志》2014年第2期513-516,共4页Journal of Experimental Hematology

基　　金：浙江省医药卫生科学研究基金项目(2010KYA055、2011ZDA005、2012KYA061)

摘　　要：本研究旨在区分人类白细胞抗原(HLA)-C*04:01:01G组内等位基因,并分析其与HLA-A、-B的连锁情况。通过收集HLA-C*04:01:01G组标本,采用多聚酶链反应序列分型法(PCR-SBT)分析HLA-C座位第2-7外显子序列,采用PCR-SBT方法对HLA-A、-B、-DRB1和-DQB1基因进行分型。结果表明:在189例HLA-C*04:01:01G组标本中,178例(94.2%)为HLA-C*04:01,11例(5.8%)为C*04:82;共检出HLA-C*04:01:01和C*04:82相关单体型72条,频率大于0.0050的单体型为26条;连锁分析显示HLA-C*04:01:01与A*02:03,A*02:07,A*11:01,A*33:03,B*13:01,B*15:01,B*15:05,B*15:27,B*40:01,B*54:01关联,而HLA-C*04:82与B*40:01关联。结论:HLA-C*04:01:01G组中存在HLA-C*04:01:01和HLA-C*04:82,在常规分型指定中应加以鉴别。The aim of this study was to investigate the relatively frequencies of alleles in the HLA-C ＊ 04：01：01G group and to analyze their relations with HLA-A and -B loci, DNA samples previously typed as HLA-C ＊ 04：01：01G were sequentially selected. The sequences for exon 2 to 7 of the HLA-C locus were analyzed by polymerase chain reaction sequence-based typing（ PCR-SBT）. The HLA-A, -B, -DRB1 and -DQB1 loci were genotyped using PCR-SBT method. The results showed that 178 samples （94.2%） and 11 samples （5, 8% ） were assigned as HLA-C ＊ 04：01：01 and HLA-C ＊ 04：82 respectively among 189 samples previously typed as HLA-C ＊ 04：01：01G. 72 haplotypes associated with HLA-C ＊ 04：01-01 and C ＊ 04：82 were found, in which the frequencies of 26 haplotypes were over 0.0050, HLA- C ＊ 04：01：01 was strongly related with A ＊ 02：03, A ＊ 02：07, A ＊ 11：01, A ＊ 33：03, B ＊ 13：01, B ＊ 15：01, B ＊ 15 ： 05, B ＊ 15：27, B .40：01, B .54：01 alleles, while HLA-C ＊04：82 was related with B ,40：01. It is concluded that HLA-C .04：01：01 and HLA-C ＊ 04：82 alleles were confn＇med in the HLA-C ＊ 04：01.01G group, which should be discriminated by the routine HLA genotyping.

关 键 词：HLA HLA—C＊04 01 01 HLA—C＊04 82 

分 类 号：R457.1[医药卫生—治疗学]