α7nAChR参与生理浓度糖皮质激素的抗炎作用  

α7nAChR is involved in anti-inflammation of physiological concentrations of glucocorticoids

作　　者：陈依雨[1,2] 吕俊华[2] 张梅[1] 

机构地区：[1]广州医科大学药学院药理教研室,广州广东510082 [2]暨南大学药学院药理教研室,广州广东510630

出　　处：《中国病理生理杂志》2014年第4期706-710,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.S2011040003942);广州市教育局基金资助项目(No.10A178)

摘　　要：目的:探讨α7烟碱型乙酰胆碱受体(α7nAChR)在生理浓度糖皮质激素(GCs)抗炎过程中的作用。方法:MTT法检测不同浓度氢化可的松对小胶质细胞BV-2活性的影响;在建立LPS刺激的BV-2细胞炎症模型基础上,实验分组如下:(1)空白对照组;(2)LPS组;(3)GCs+LPS组;(4)α7nAChR阻断剂甲基牛扁亭碱(MLA)+GCs+LPS组,ELISA法测定细胞上清中TNF-α和IL-1β的含量。结果:2 000和1 000 nmol/L氢化可的松可分别使细胞存活率降低至(76.9±5.5)%和(90.8±7.3)%,表现出超生理剂量GCs的细胞损伤作用。LPS明显刺激BV-2细胞释放TNF-α和IL-1β,并呈现时间和剂量依赖性。生理浓度(500和250 nmol/L)的氢化可的松均可减少LPS诱导BV-2细胞释放TNF-α和IL-1β,10 nmol/L MLA预处理BV-2细胞能拮抗GCs抑制炎症因子释放的作用。结论:α7nAChR参与了生理浓度GCs的抗炎作用。AIM： To explore the role of or7 nicotinic acetylcholine receptor （ct7nAChR） in anti-inflammation of glucocorticoids （GCs） at physiological concentrations. METHODS： MTY assay was used to measure the viability of BV-2 ceils, which were processed by hydrocortisone at different concentrations. On the basis of inflammatory model induced by LPS in BV-2 cells, experimental groups were divided as follows ： （ 1 ） control; （2） LPS; （3） GCs ＋ LPS; （4） methyl- lycaconitine （MLA） ＋ GCs ＋ LPS. The levels of TNF-a and IL-1B in the cell supernatants were detected by ELISA. RE- SULTS ： Hydrocortisone at concentrations of 2 000 and 1 000 nmol/L decreased the cell viability to （76.9 ± 5.5 ） % and （90.8 ± 7.3 ） %, respectively, indicating the cellular injury by GCs at over-physiological doses. LPS significantly induced the releases of TNF-a and IL-113 in a time- and dose-dependent manner in BV-2 cells. Hydrocortisone at physiological con- centrations （500 and 250 nmol/L） reduced the releases of TNF-a and IL-1B in BV-2 cells stimulated by LPS, and MLA at concentration of 10 nmol/L antagonized the anti-inflammatory effect of GCs. CONCLUSION： ct7nAChR is involved in the anti-inflammatory effect of the physiological concentrations of GCs.

关 键 词：糖皮质激素 a7碱型乙酰胆碱受体 小神经胶质细胞 脂多糖类 

分 类 号：R338.2[医药卫生—人体生理学]