川崎病患儿细胞免疫功能状况及转录因子T-bet和GATA3的调控作用  

Functional state of T cells and role of transcription factors T-bet and GATA3 in children with Kawasaki disease

作　　者：王从军[1] 陈梅[1] 雷中劲[2] 

机构地区：[1]荆楚理工学院医学院 [2]荆门市第一人民医院儿科,湖北荆门448000

出　　处：《中国病理生理杂志》2014年第4期733-737,共5页Chinese Journal of Pathophysiology

基　　金：湖北省教育厅优秀中青年人才项目(No.Q20104302)

摘　　要：目的:研究川崎病患儿细胞免疫功能状况及转录因子T-bet和GATA3的调控作用。方法:选取41例川崎病患儿为研究对象,同时选取年龄和性别相匹配的因急性上呼吸道感染发热儿童40例为对照组,分别采用三色荧光流式细胞术和半定量逆转录-聚合酶链反应检测2组患儿外周血Th1、Th2细胞数量及单个核细胞中T-bet和GATA3 mRNA水平。结果:川崎病患儿外周血Th1、Th2细胞比率及单个核细胞转录因子T-bet mRNA、GATA3 mRNA转录水平分别为(10.36±3.69)%、(6.46±2.28)%、0.51±0.16和0.38±0.13,均显著低于对照组[(25.26±5.22)%、(16.87±4.35)%、0.62±0.21和0.46±0.12,P<0.05]。Spearman相关分析显示Th1和Th2细胞比率分别与T-bet mRNA和GATA3 mRNA水平呈正相关。结论:川崎病患儿急性期T细胞并非活化而是处于抑制状态,T-bet和GATA3分别对Th1和Th2细胞增殖具有调控作用。AIM： To clarify the functional state of T cells and the role of transcription factors T-bet and GA- TA3 in choldren with Kawasaki disease. METHODS： The patients with acute Kawasaki disease （n =41 ） were enrolled as experimental group and 40 febrile children with acute upper respiratory tract infection were selected as control group. The mRNA expression levels of T-bet and GATA3, and the percentages of Thl and Th2 cells were examined by RT-PCR and flow cytometry respectively. RESULTS： The mRNA expression levels of T-bet and GATA3 and the percentages of Thl and Th2 cells in Kawasaki disease group were 0.51 ± 0.16, 0.38 ± 0.13, （ 10.36 ± 3.69） % and （6.46 ± 2.28 ） %, respec- tively, and were significantly lower than those in control group [ 0. 62 ± 0.21, 0.46 ±0. 12, （25.26 ± 5.22 ）% and （16. 87 ±4.35 ）%, P 〈 0.05 ]. There were positive correlations between the mRNA expression levels of T-bet and GA- TA3, and the percentages of Thl and Th2 cells. CONCLUSION： Associated with the suppression of both T-bet and GA- TA3 mRNA expression, the function of Thl and Th2 cells are suppressed simultaneously in acute Kawasaki disease.

关 键 词：川崎病 细胞免疫 T-bet蛋白 GATA3转录因子 

分 类 号：R725[医药卫生—儿科]