HPLC法测定津蓟中风Ⅰ号颗粒中梓醇和五味子醇甲  

Determination of catalpol and schisandrin in Jinji Zhongfeng No. 1 Granules by HPLC

作　　者：张学明[1] 戴学文[2] 王姿婧[2] 刘青[2] 房志仲[2] 

机构地区：[1]天津市蓟县中医院药剂科,天津301900 [2]天津医科大学药学院药剂学教研室天津市临床药物关键技术重点实验室,天津300070

出　　处：《现代药物与临床》2014年第4期385-388,共4页Drugs & Clinic

摘　　要：目的建立津蓟中风I号颗粒中梓醇和五味子醇甲的HPLC测定方法。方法采用HPLC法分别对梓醇、五味子醇甲进行测定。梓醇的流动相为乙腈-0．1％磷酸（1：99），体积流量为1．0mL／min，检测波长为210nm。五味子醇甲的流动相为甲醇一水（75：25），体积流量为0．8mL／min，检测波长为250nm。结果梓醇在4．25~68．00μg／mL与峰面积线性关系良好，平均回收率为100．22％，RSD值为1．05％（n=9）。五味子醇甲在4．0～32．0rtg／mL与峰面积线性关系良好，平均回收率为100．49％，RSD值为1．25％（n=9）。结论本法简便、准确性高、灵敏度高、专属性和重现性好，可作为津蓟中风I号颗粒中梓醇和五味子醇甲的测定方法。Objective To establish a method for the determination of schisandrin and catalpol in Jinji Zhongfeng No. 1 Granules. Methods Catalpol and schisandrin were determined by HPLC method, respectively. Catalpol was determined with acetonitrile - 0.1% phosphoric acid （1 ： 99） as the mobile phase, the flow rate was 1.0 mL/min, and the detection wavelength was 210 nm. Schisandrin was determined by methanol - water （75： 25） as the mobile phase, the flow rate was 0.8 mL/min, and the detection wavelength was 250 nm. Results The liner ranges of catalpol and schisandrin were 4.25 -- 68.00 pg/mL （r=0.999 9）, 4.00 -- 32.00 μg/mL （r=0.999 9）. The average recoveries were 100.22%,and 100.49%, with RSD values of 1.05%,and 1.25% （n=9）, respectively. Conclusion The method is specific, accurate and reproducible, and can be used for the quality control of catalpol and schisandrin for Jinji Zhongfeng No. 1 Granules.

关 键 词：津蓟中风I号颗粒 梓醇 五味子醇甲 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]