外源腐胺对干旱胁迫下苹果幼苗叶绿素荧光等生理指标的影响  

Effects of exogenous putrescine on chlorophyll fluorescence and other physiological indexes of apple seedling under water stress

作　　者：张媛[1] 郭兴科[1] 李中勇[1] 徐继忠[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001

出　　处：《河北农业大学学报》2014年第2期40-44,共5页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金资助项目(303205);国家苹果产业技术体系(CARS-28)

摘　　要：采用盆栽控水方式,设置2个干旱胁迫水平(轻度和重度),每个胁迫水平各设3个腐胺(Put)浓度(1×10-4,1×10-5,0mol/L),研究干旱胁迫下外源腐胺对苹果幼苗叶绿素荧光、叶绿素含量和超氧化物歧化酶(SOD)活性的影响。结果表明:外源Put提高干旱胁迫下PSⅡ最大光化学效率(Fv/Fm),缓解中午强光下光抑制的发生;Put处理提高了SOD活性和叶绿素含量,1×10-5,1×10-4 mol/L处理,11d时SOD酶活性分别增加了5%,12%(轻度)和13%,22%(重度),19d时叶绿素含量分别增加了11%,23%(轻度)和20%,33%(重度)。表明外源Put可减轻干旱胁迫对光合机构的伤害,提高苹果幼苗的抗旱性,本试验中1×10-4 mol/L处理效果为佳,且在重度胁迫下效果更明显。The purpose of this study was to investigate the effects of exogenous putrescine （Put） on chlorophyll fluorescence, chlorophyll contents and superoxide dismutase activities of apple seedling under water stress. Using pot-culture experiments simulating soil water status, two levels of water stress（low and severe） and three Put concentrations （1 × 10-4 tool/L, 1 × 10-5 and 0 tool/L） for each water level were designed. The results showed that the PS Ⅱ photo- chemical quantum yield （Fv/Fm） was increased, but photoinhibition during midday was de- creased in Put treatments under water stress. Furthermore, Put treatments improved the activ- ity of SOD, as well as alleviated chlorophyll degradation. In 1× 10-5 mol/L and 1 × 10-4 mol/L treatments, the activities of SOD increased by 5% and 12＆ under low stress and 13%and 22% under severe stress respectively on the llth day, and the contents of chlorophyll increased by 11% and 23% under low stress and 20% and 33% under severe stress respectively on the 19th day comparing with the control. These results indicated that the application of exogenous Putcould alleviate drought-induced sistance of apple seedling. The seeding to water stress and this damage 1 × 10-4 relieve on photosynthetic apparatus and enhance drought re- mol/L treatment could enhance the tolerance of apple effects under severe stress was obvious.

关 键 词：腐胺 干旱胁迫 叶绿素荧光参数 苹果 

分 类 号：S661.1[农业科学—果树学]