毛醌素(Piloquinone)生物合成基因簇的初步鉴定  

The preliminary identification of piloquinone biosynthetic gene cluster

作　　者：张学文[1,2] 殷华[2] 周威[1,2] 庄以彬[2] 张同存[1] 刘涛[2] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国科学院天津工业生物技术研究所,天津300308

出　　处：《河北农业大学学报》2014年第2期76-81,共6页Journal of Hebei Agricultural University

基　　金：天津市科技支撑计划重点项目(11ZCKFSY07900)

摘　　要：通过对毛链霉菌(Streptomyces pilosus)进行基因组测序及生物信息学分析,发现基因组中分布了26个次级代谢物合成基因簇;并通过构建敲除质粒分别破坏了2个Ⅱ型聚酮化合物基因簇7pil和8pil中Ⅱ型聚酮合酶核心酶KSα和KSβ基因,获得敲除株pil1415和pil4243;通过HPLC检测发现敲除株pil1415代谢产物中仍能检测到毛醌素的产生,而敲除株pil4243的代谢产物中检测不到毛醌素的产生,表明8pil中Ⅱ型聚酮化合物基因簇中核心酶基因的敲除阻断了毛醌素的生物合成,确定Ⅱ型聚酮化合物基因簇8pil是毛醌素的生物合成基因簇。Based on the genome sequencing and bioinformatic analysis, biosynthetic gene clus- ters of 26 secondary metabolites were found in genome of Streptomyces pilosus. 7pil and 8piI were two Type Ⅱ PKS gene clusters, which might be involved in piloquinone biosynthesis. The genes of KSα, KSβ in 7pil and 8pil were disrupted respectively, and the corresponding mutants of pi11415 and pil4243 were gained and identified. The mutant of pi14243 had no de- tectable piloquinone production during fermentation, and which showed gene cluster 8pil is re- sponsible for piloquinone biosynthesis.

关 键 词：毛链霉菌(Streptomyces pilosus) 毛醌素 基因组挖掘 Ⅱ型聚酮合酶 

分 类 号：Q591.9[生物学—生物化学]