抗原肽修饰的鸡法氏囊病毒VP_2蛋白的制备及其免疫活性  

The development of infectious bursal disease virus VP_2 protein modified with epitope peptide and its immune activity

作　　者：蔡梅红[1] 杨禄[1] 谭仁可 桂国建[1] 杜坤鹏[1] 王亮[1] 马海乐[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《河北农业大学学报》2014年第2期82-86,共5页Journal of Hebei Agricultural University

基　　金：江苏省博士后基金(1202042C);江苏大学高级人才启动基金资助(12JDG099);江苏大学科研立项项目(Y12A012)

摘　　要：为了分析抗原肽修饰的鸡法氏囊病毒VP2蛋白的免疫活性,本试验设计2条引物,以扩增N端和C端各含有一个B细胞抗原肽密码子的鸡法氏囊病毒VP2的核酸序列(mVP2)。通过EcoR I和XhoI两个酶切位点使该核酸序列插入原核表达载体(PET32a)。SDS-PAGE试验结果表明,重组鸡法氏囊病毒mVP2蛋白在大肠杆菌中的表达量为8.5%。琼脂糖扩散试验(AGP)的结果表明,重组鸡法氏囊mVP2蛋白的琼扩效价为1∶16。表明抗原修饰的鸡法氏囊病毒VP2蛋白具有明显的体外免疫学活性。In order to analyze the immunity activity of infectious bursal disease virus VP2 modi- fied with epitope epitopes, two primers were designed to amplify infectious bursal disease virus VPz gene modified with one B cell epitope at the N terminal and another B cell epitope at the C terminal （mVP2）. The nucleic acid sequence cleaved by EcoR I and Xho I was inserted into the prokaryotic expression vector pPET32a. SDS-PAGE results showed that recombinant infec- tious bursal disease virus mVP2 protein comprised about 8. 5% of the E. coli total protein. Agarose diffusion assay （AGP） results showed that AGP titer of recombinant IBDV mVP2 is up to 1 ： 16. Above results suggested that VP2 protein modified with epitope epitopes has sig- nificant immune activity in vitro.

关 键 词：法氏囊病毒 重组mVP2 免疫活性 

分 类 号：S858.31[农业科学—临床兽医学]