经皮三叉神经慢性电刺激对慢性癫痫大鼠海马和皮层内VGLUT1表达的影响  

Effects of chronic percataneous trigeminal nerve stimulation on the hippocampal and cortical expression of VGLUT1 in chronic epileptic rats

作　　者：贺慧艳[1] 左健[1] 王倩倩[1,2] 尹娜[1,3] 刘益民[4] 王玉[1] 

机构地区：[1]安徽医科大学第一附属医院神经内科,安徽合肥230022 [2]安徽医科大学解放军临床学院/解放军第105医院神经内科,安徽合肥230011 [3]淮北职业技术学院医学系,安徽淮北235000 [4]绍兴第二医院,浙江绍兴312000

出　　处：《中风与神经疾病杂志》2014年第4期295-298,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.81271444);安徽省战略性新兴产业科技攻关项目(No.11010402166)

摘　　要：目的观察经皮三叉神经慢性电刺激(trigeminal nerve stimulation,TNS)对匹罗卡品诱导的癫痫大鼠海马和皮层内囊泡型谷氨酸转运体-1(vesicular glutamate transporter 1,VGLUT1)表达的影响,探讨TNS治疗癫痫的可能机制。方法将达到癫痫持续状态(status epilepticus,SE)的实验大鼠随机分为模型(Pilo)组和经皮三叉神经慢性电刺激(TNS)组;正常对照组给予同剂量的生理盐水代替Pilo腹腔注射,随后对照组和Pilo组均给予为期一个月的经皮三叉神经假性电刺激(TNS)组给予为期一个月的真性电刺激。通过免疫荧光双标和Western blot分析海马和皮层内VGLUT1的表达。结果 TNS组和Pilo组海马、皮层内VGLUT1的表达均呈先升高后下降的趋势,约在72 h左右达到高峰。在24 h时,TNS组海马和皮层内VGLUT1的表达均明显高于Pilo组(P<0.01),而在以后各个时间点均低于Pilo组(P<0.05,P<0.01)。结论经皮三叉神经慢性电刺激能够减少癫痫大鼠脑内VGLUT1的表达,从而影响谷氨酸能神经元兴奋性神经递质的传递,推测TNS抗癫痫的机制可能与此有关。Objective To investigate the effects of chronic percataneous trigeminal nerve stimulation （TNS） on the hippocampal and cortical expresssion of vesicular glutamate transporter 1 （ VGLUT1 ） in rats with chronic epilepsy to ex plore the possible antiepileptic mechanisms of TNS. Methods SE rats, introduced by pilocarpine, were randomly divided into two groups：Pilo group in which the rats were treated with sham TNS for one month;and TNS group in which the rats treated with TNS for one month. Control group rats were treated with sham TNS for one month after physiological saline was intraperitoneally injected. The expression of VGLUT1 in hippocampus and cortex was examined with double immunofluores cence and western blot. Results The expression of VGLUT1 in the hippocampus and cortex reached peak at 72 h postTNS ascended before 72 h but descended later in Pilo as well as in TNS group rats. The hippocampal and cortical expression of VGLUT1 was higher at 24 h postTNS（ P 〈 0.01 ）, but lower （P 〈 0.05, P 〈 0.01 ） at other different timepoints in TNS group rats than that in Pilo group rats. Conclusion TNS may exert its antiepileptic function through regulating excitatory synaptic transmission in the cortex and hippocampus of epileptic brain.

关 键 词：癫痫 三叉神经电刺激 VGLUT1 匹罗卡品 免疫荧光 Western BLOT 

分 类 号：R565[医药卫生—呼吸系统]