补肾活血颗粒的纯化工艺优选  

Optimization of Purification Technology of Bushen Huoxue Granule

作　　者：尹华[1] 李渊贞[1] 章建华[2] 

机构地区：[1]浙江中医药大学药学院药标准化研究实验室,浙江杭州310053 [2]浙江中医药大学第一临床医学院,浙江杭州310006

出　　处：《中华中医药学刊》2014年第5期967-970,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中药现代化计划项目(第一批)(浙经信[2010]421号)

摘　　要：目的:优选补肾活血颗粒的纯化工艺。方法:采用单因素试验法和L9(34)正交试验法,考察乙醇浓度、醇沉时间等因素对纯化工艺的影响,以羟基红花黄色素A和松脂醇二葡萄糖苷的转移率为评价指标,采用多指标综合评分法优选最佳的纯化工艺。结果:补肾活血颗粒最佳纯化工艺为减压浓缩至药液相对密度为1.05,加95%乙醇使含醇量为60%,醇沉24 h。结论:优选得到的纯化工艺方法简便可靠,重复性好,有效成分转移率高,可以保证补肾活血颗粒的质量和疗效。Objective ： To optimize the purification technology of Bushen Huoxue Granule. Method： By using single fac- tor and orthogonal test of L9 （34）, the effects of ethanol concentration, ethanol precipitation time and other factors were investigated. With the content of the transfer rate of Safflomin A and Pinoresinol diglucoside as evaluation indexes, the multi - index comprehensive evaluation method was used to optimize the purification technology of Bushen Huoxue Gran- ule. Results： The optimal purification technology of Bushen Huoxue Granule was as：the extracts were condensed to a rela- tive density of 1.05 and participated by 95% alcohol at the concentration of 60% and then stewing tor 24 h. Conclusion： The optimized purification technology is simple and reliable and has good reproducibility and high efficient, and can guar- antee the quality and effect of Bushen Huoxue Granule.

关 键 词：补肾活血颗粒 纯化工艺 

分 类 号：R284.1[医药卫生—中药学]