臭豆碱在大鼠肾脏中的分布及其毒动学  

Distribution and Toxicokinetics of Oral Anagyrine in Kidney of Rats

作　　者：达林其木格[1] 李培锋[2] 关红[2] 

机构地区：[1]呼和浩特职业学院,内蒙古呼和浩特010050 [2]内蒙古农业大学动物医学与科学院,内蒙古呼和浩特010018

出　　处：《中华中医药学刊》2014年第5期1009-1011,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(39960060)

摘　　要：目的:按6.82 mg/(kg·bw)大鼠单剂量口服臭豆碱,用反相离子对高效液相色谱方法,测定大鼠肾组织中不同时间点臭豆碱的浓度。方法:高效液相色谱法,色谱柱为大连产HYP-ODS柱(200 mm×4.6 mm,5μm),柱温30℃;流动相由水相-异丙醇-乙腈(150∶15∶20体积比)组成。水相部分每1000 mL中含SDS 0.5g,三乙胺3.5 mL,85%磷酸2 mL(用三乙胺调至PH 3.0);流速1.0 mL·min-1紫外检测波长309 nm。结果:经DASver1.0药代动力学软件处理数据,臭豆碱在肾组织符合一室模型,其动力学参数为:Tmax(60)min,Cmax(13747.80)μg/L,T1/2(113.34)min,T1/2ka(16.19)min,AUC(3033978)μg/L·min,Vd/F(16.193 L/kg),CL/F(0.005)L/min/kg。结论:臭豆碱灌服后,可迅速分布到肾脏,具有消除快、蓄积时间短的特点。Objective ： To measure the concentration of Anagyrine in its liver at different times by the way of an ion - pair liquid chromatographic （IPLC） by giving the rat a dose of 6.82 mg/（ kg · b · w） orally. Method： The liquid chrom- atographic analysis was performed on a （LC - 10A） HPLC system （SHIMADZU）, consisting of（ LC - 10AT） pump. Separations were accomplished with a HYP -ODS C18 （200 mm x4.6 mm,5 μparticle size） from Dalian （China） ,op- erating at 30℃. The mobile phase consisted of water phase - isopropanol - acetonitrile （ 150 ： 15 ： 20） （v/v）. Every 1000 mL of water phase contained 0.5 g of SDS ,3.5 mL of triethylamine and 2 mLof 85% phosphoric acid （ pH of mobile phase adjusted to 3.0 by addition of triethylamine）. The mobile phase was filtered before using 0.45 μm nitrocellulose membrane and the flow rate was 1.0 mL · min-1 The UV detection wavelength was 309 nm. The detection limits of anagyrine was 10 μg · L^ - 1 （ S/N = 3 ）. Results ： The statistics was processed by a software dealing with medication dy- namics named DASverl. 0. It was found that the persistence pattern of Anagyrine in the liver was in agreement with the one - chamber model and its dynamic parameters were Tmax （ 60 ） rain, Cmax （ 13747.80 ） ） ng/g, TI/2 （ 113.34 ） min, T1/2k. （ 16.19）min, AUC（3033978） ng/（ g · min）, Vd/F （ 16. 193L/kg）, CL/v （0. 005 ） L/（ min· kg）. Conclusion： After a dose of Anagyrine is given,it diffuses through the blood vessels into the kidney Immediately. It tends to vanish quickly without any remnant.

关 键 词：臭豆碱 反相离子对高效液相色谱法 毒动学 组织分布 

分 类 号：R285.5[医药卫生—中药学]