醒脑液对PAP双转基因痴呆小鼠NEP和BACE1 mRNA表达的影响  

Effect of XNY on Neprilysin and BACE1 mRNA Expression in Alzheimer's Disease of PAP Double Transgenic Mice

作　　者：张子洋 常富业 张允岭[2] 王新祥[2] 郑宏[2] 马涛[2] 刘雪梅[2] 闫妍[2] 单媛莉 

机构地区：[1]煤炭总医院老年病科,北京100028 [2]北京中医药大学东方医院实验中心,北京100078

出　　处：《中华中医药学刊》2014年第5期1016-1018,I0007,I0008,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81072851)

摘　　要：目的:探讨中药醒脑液(XNY)对PAP双转基因痴呆小鼠大脑组织中中性内肽酶(NEP)和β分泌酶(BACE1)mRNA表达的影响。方法:采用实时荧光定量PCR技术分别观测正常对照组、模型组、西药对照组、XNY高剂量组、XNY中剂量组和XNY低剂量组小鼠大脑组织中NEP和BACE1 mRNA的表达。结果:与模型组相比,XNY高剂量组脑内NEP mRNA含量表达上调(P<0.05);XNY高、中剂量组BACE1 mRNA含量表达下降(P<0.01)。结论:醒脑液对双转基因痴呆小鼠大脑组织中NEP和BACE1 mRNA表达有较好的调控作用,说明醒脑液对防治老年性痴呆能起到良好效果。Objective ：TO observe the effect of XNY on neprilysin and BACEI mRNA expression in brain tissue of PAP double transgenic mice. Methods ： By real - time fluorescent quantitative PCR technique, the NEP and BACE1 mRNA of double transgenic mice were observed in normal control group, model group, positive control group, high dose group, medi- um dose group and low dose group. Results：Compared with the model group, the mRNA content of NEP could be in- creased by high dose group （P 〈 0.05） and the mRNA content of BACE1 could be reduced by high and medium dose groups. （P 〈 0.01 ）Conclusion：XNY has good regulation effects on NEP and BACE1 mRNA expression in brain tissue of PAP double transgenic mice. Its account for the XNY can play a good effect for prevention and control of senile dementia.

关 键 词：醒脑液 PAP双转基因小鼠 中性内肽酶 Β分泌酶 阿尔茨海默病 老年性痴呆 基因表达 

分 类 号：R285.5[医药卫生—中药学]