“肝郁气滞”及“肝郁脾虚”型抑郁症动物模型建立初探  

Study of Mice Models of Liver-Qi Stagnation and Liver-Qi Stagnation with Spleen Insufficiency Depression

作　　者：周国儿[1] 吴静[2] 黄云娟[1] 陆红[2] 

机构地区：[1]浙江省舟山医院,浙江舟山316000 [2]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2014年第5期1035-1038,I0010,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科学研究基金项目(2011ZB157)

摘　　要：目的:建立中医"肝郁气滞"型抑郁症、"肝郁脾虚"型抑郁症动物模型。方法采用慢性不可预见性应激加孤养法、束缚法、束缚加喂食食醋法建立传统抑郁症、"肝郁气滞"型抑郁症、"肝郁脾虚"型抑郁症动物模型,并考察了小鼠一般状况、体重变化、强迫游泳实验、开阔场实验以及自主活动等行为学指标的变化。结果造模组、气滞组和脾虚组较空白组小鼠均出现出现懈怠懒动、皮毛粗糙等现象。第35 d空白组同其他3组、模型组同气滞组、脾虚组体重变化均有极显著性差异(P<0.01);强迫游泳实验表明,空白组、模型组同其他各组均有极显著性差异(P<0.01);开阔场实验表明,空白组同其他各组的爬行格数有极显著性差异(P<0.01)。空白组同脾虚组的中央格停留时间有极显著性差异(P<0.01)。同时,空白组同气滞组、模型组同脾虚组均有显著性差异(P<0.05)。自主活动实验表明,空白组同模型组、气滞组,模型组同脾虚组的自主活动次数有极显著性差异(P<0.01)。结论本研究成功建立传统抑郁症、"肝郁气滞"型抑郁症、"肝郁脾虚"型抑郁症动物模型。四项行为学指标显示,中医不同证候抑郁症动物模型与传统抑郁症动物模型相比有显著性差异,为进一步建立科学的中医证候抑郁症模型提供参考依据。Objective ： To establish and evaluate the mice model of the liver - Qi stagnation syndrome and the liver - Qi stagnation with spleen insufficiency syndrome for depression. Methods ： The mice models of traditional depression, liver - Qi stagnation of depression,liver - Qi stagnation with spleen insufficiency were established by the chronic unpredictable mild stress intervention, chronic binding, chronic binding up with drinking acetic acid separately. The change of macro- scopic token, body weight, the open - field test, the forced swimming test and the autonomic activity were observed. Results ： There were abnormal behaviors among the model group, liver - Qi stagnation group and spleen insufficiency group, such as slackness, loose tools, matte fur. After 35 days, the blank control group compared with the other three groups and the model group compared with the liver - Qi stagnation group and the spleen insufficiency group had a signifi- Cantly decrease in body weight （ P 〈 O. Ol ）. The forced swimming test suggested that the blank control group compared with the other three groups and the model group compared with the liver - Qi stagnation group and the spleen insufficiency group had a significantly extending （ P 〈 0.01 ）. In the open - field test, the blank control group compared with the other three groups had a significantly difference in the number of square in creeping （ P 〈 0. 01 ）. The blank control group compared with spleen insufficiency group had a significantly difference in the stop time in the middle position （ P 〈 0. 01 ） and the blank control group compared with liver - Qi stagnation group and the model group compared with spleen insufficiency group had an obvious difference in the stop time in the middle position （ P 〈 0. 05 ）. The autonomic activity test suggested that the blank control group compared with the model group and the liver - Qi stagnation group and the model group compared with the spleen insufficiency group had a significantly difference （ P 〈

关 键 词：抑郁症 中医证候模型 肝郁气滞 肝郁脾虚 行为学观察 

分 类 号：R285.5[医药卫生—中药学]