大孔吸附树脂纯化黄连中4种生物碱的含量测定  

Determination of Four Kinds of Alkaloids in Coptis Chinensis Purified by Macroporous Absorption Resin

作　　者：田原[1] 于艳[1] 陈雪[2] 闫冬[2] 徐晶[2] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110847 [2]辽宁中医药大学,辽宁沈阳110032

出　　处：《中华中医药学刊》2014年第5期1060-1062,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅重点实验室项目(LS2010105)

摘　　要：目的:建立反相高效液相色谱法同时测定大孔吸附树脂富集纯化后的黄连提取物中4种生物碱含量的方法。方法:以X-5型大孔吸附树脂对黄连提取物中总生物碱进行富集纯化,采用高效液相色谱法同时测定其中表小檗碱、黄连碱、巴马汀和小檗碱的含量。结果:黄连提取物经大孔吸附树脂纯化后,表小檗碱、黄连碱、巴马汀和小檗碱的平均含量分别为1.45%、2.62%、7.03%和17.95%,未经纯化的黄连提取物中的平均含量分别为0.67%、1.05%、1.86%和8.54%。结论:X-5型大孔吸附树脂可有效富集纯化黄连中的生物碱类成分。Objective ： To establish a method for determining the contents of four kinds of alkaloids in Coptis chinensis purified by macroporous absorption resin by Rp - HPLC. Methods ： The macroporous absorption resin X - 5 was used to separate and purify the total alkaloids from Coptis chinensis. The HPLC method was used to determine the contents of epiberberine, eoptisine, palmatine and berberine. Results ： The average contents of epiberberine, eoptisine, palmatine and berberine in Coptis chinensis with macroporous absorption resin were 1.45% ,2.62% ,7.03% ,17.95% and the average contents without macroporous absorption resin were 0.67%, 1.05%, 1.86% and 8.54%. Conclusion：The alkaloids in Coptis ehinensis can be effectively separated and purified with the maeroporous absorption resin X -5.

关 键 词：HPLC 大孔吸附树脂 表小檗碱 黄连碱 巴马汀 小檗碱 

分 类 号：R284.1[医药卫生—中药学]