涤痰开窍方对卒中后抑郁症大鼠海马BDNF蛋白表达的影响  

Influence of Ditan Kaiqiao Formula on BDNF Protein in Hippocampus of Post-Stroke Depression Rat

作　　者：张伟骏[1] 陈眉[1] 

机构地区：[1]浙江中医药大学第一临床医学院,浙江省中医院,浙江杭州310006

出　　处：《中华中医药学刊》2014年第5期1099-1100,I0010,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药管理局项目(2005C026);浙江省卫生厅项目(2013KYA139)

摘　　要：目的:建立脑卒中后抑郁症(post-stroke depression,PSD)的大鼠模型,观察海马脑源性神经营养因子(BDNF)的损伤情况,并从分子生物学角度研究中药涤痰开窍方对脑卒中后抑郁症大鼠海马区BDNF蛋白表达的影响及神经保护作用。方法:采用双侧颈总动脉永久性结扎致大鼠慢性低灌注PSD模型,研究涤痰开窍方对脑卒中后抑郁症大鼠的治疗作用,采用用免疫组化法检测大鼠海马BDNF蛋白的表达。结果:与模型组比较,涤痰开窍方能使海马C3区BDNF蛋白表达增多(P<0.01或<0.05)。结论:中药涤痰开窍方的治疗能上调卒中后抑郁症大鼠海马BDNF蛋白,从而起到保护神经元的作用。Objective ： To establish a rat model of post - stroke depression and observe the damage of hippocampal neu- rons and brain -derived neurotrophic factor （BDNF） in the hippocampal area. To investigate the protection effects of Di- tan Kaiqiao formula on hippocampal neuron of post -stroke depression （PSD） rat from molecular biology and the influ- ence on BDNF protein. Methods ： A rat model of chronic hypoperfusion post - stroke depression was induced by permanent bilateral carotid artery ligation and the therapeutic effects of Ditan Kaiqiao formula on PSD rat hippocampal BDNF protein expression was studied by immunohistochemical method. Result ： Compared with the model group, Ditan Kaiqiao formula could significantly raise the expression of BDNF in the hippocampal area （P 〈 0.01 or P 〈 0. 05 ）. Conclusion ： Ditan Kaiqiao formula could conserve neuron on post - stroke depression （PSD） rat by raising the expression of BDNF in the hippocampal area.

关 键 词：卒中后抑郁症 涤痰开窍方 海马 BDNF 

分 类 号：R285.5[医药卫生—中药学]