养血活血方对便秘老年小鼠肠神经递质的影响  

Effects of Yangxue Huoxue Recipe on Intestinal Neurotransmitters in Old Rats with Colonic Constipation

作　　者：王辰[1] 杨关根[2] 赵蕾[3] 刘智勇[2] 沈忠[2] 杨琴燕[2] 王东[3] 邓群[2] 姚江颖[2] 张秀峰[2] 

机构地区：[1]浙江中医药大学第二临床医学院,浙江杭州310053 [2]杭州市第三人民医院肛肠外科,浙江杭州310009 [3]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2014年第5期1137-1139,I0016,I0017,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(Y2110782);浙江省科技厅公益技术研究社会发展项目(2012C33097);杭州市科技局医疗卫生重点专科专病项目(20120533Q06)

摘　　要：目的:研究养血活血方对便秘老年小鼠肠神经递质的影响,阐明养血活血方治疗老年性便秘的作用机理。方法:选取健康老年小鼠,采用吗啡复制便秘小鼠模型,造模成功后,将60只健康老年ICR小鼠随机分为6组:空白组、模型组、阳性对照组(麻仁软胶囊)、养血活血方高、中、低剂量组。各组分别给药,连续灌服3周后处死,取结肠组织。采用免疫组化方法测定肠神经递质SP、VIP、5-HT的表达。结果:与空白组、阳性对照组及养血活血方各剂量组相比,模型组肠壁组织内SP、VIP、5-HT阳性表达明显减少,差异均有显著性统计学意义(P<0.01);各给药组组间比较可见:养血活血方高、中剂量组的神经递质SP、VIP、5-HT含量高于养血活血方低剂量、阳性对照组,差异有统计学意义(P<0.05或P<0.01)。结论:养血活血方可能通过增加肠神经递质SP、VIP、5-HT阳性表达来起到通便的作用,并且养血活血方的通便作用呈一定的量效依赖关系。Objective ： To study the effects of Yangxue Huoxue Recipe （YXHYR） on intestinal neurotransmitters in old rats with colonic constipation, and to clarify the mechanism of Yangxue Huoxue Recipe in relieving elder constipation. Methods：The animal model with colonic constipation was established by old rats from subcutaneous injection with Mor- phine. 60 old rats with colonic constipation （slow transit colon） were divided into 6 groups：controL group, untreated group,Maren Soft Capsules - treated group, treatment group with high dosage YXHYR （ H - YXHYR）, treatment group with middle dosage YXHYR （M -YXHYR）, and treatment group with low dosage YXHYR （L- YXHYR）. Each group was administered. 3 weeks, and then the colon specimen was obtained. Intestinal neurotransmitters including substance P （ SP）, Vasoactive intestinal peptide （VIP） and 5 - HT were detected by immunohistochemistry method. Results： The ex- pressions of SP, VIP and 5 - HT in the untreated group were lower than those of the other groups （P 〈 0.01 ）. Compared with the Maren Soft Capsules - treated group and L - YXHYR group, the expressions of SP, VIP and 5 - HT in the H - YXHYR and M -YXHYR groups were increased obviously （P 〈 0.05 or P 〈 0. 01 ）. Conclusion： Yangxue Huoxue Reci- pe could have a purge role, and this may be due to its effect of regulating the expressions of SP, VIP and5 - HT in the in- testinal neurotransmitters, which shows a dose -effect relationship.

关 键 词：养血活血方 慢性便秘 肠神经递质 

分 类 号：R285.5[医药卫生—中药学]