补肾健脾中药对后巩膜加固术后兔后巩膜内bFGF浓度分布的影响  

Effect of Concentration Distribution of bFGF by Using TCM Prescription for Tonifying Kidney and Spleen in Posterior Scleral Reinforcement of Rabbit Eyes

作　　者：闫冉[1] 刘求红[2] 彭明欢[1] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中华中医药学刊》2014年第5期1140-1142,I0017,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省科技厅社会发展项目(2010B030700070)

摘　　要：目的:研究补肾健脾中药对后巩膜加固术后家兔后巩膜中碱性成纤维细胞生长因子(bFGF)浓度分布的影响,从分子生物学角度研究补肾健脾中药对后巩膜加固术后巩膜组织重塑的影响,进而研究后巩膜加固术结合补肾健脾中药防治高度近视的机理。方法:将成年新西兰白兔12只随机分为空白对照组,单纯手术组(后巩膜加固术组),手术配合中药1组(后巩膜加固配合近视Ⅰ号方组),和手术配合中药2组(后巩膜加固配合近视Ⅱ组)。于术后12周处死部分白兔,摘出右眼球,冲洗干净,切取植片及其附着的巩膜,通过细胞免疫组织化学方法,分析术后白兔后巩膜内bFGF浓度变化。结果:空白组巩膜中bFGF浓度分布非常稀疏,增殖很少;单纯手术组术后初期表现为强烈的炎症反应,有大量炎症细胞浸润,bFGF浓度稀疏,增殖较少;手术配合中药组术后炎症反应较单纯手术组明显较轻,少量炎症细胞浸润,bFGF浓度稠密,大量增殖;手术配合中药1组和手术配合中药2组bFGF浓度分布未见明显差异,均较空白组及单纯手术组密集。结论:后巩膜加固术配合补肾健脾中药能更有效地促进巩膜中bFGF的增殖,从而达到治疗高度近视的作用。Objective：To study the effect on distribution concentration of bFGF by using Chinese herbal medicine pre- scription for tonifying kidney and spleen in posterior scleral reinforcement of rabbit eyes. From the perspective of molecu- lar biology to study the influence of the scleral reinforcement postoperative scleral tissue remodeling by using Chinese herbal medicine prescription for tonifying kidney and spleen and study the mechanism of prevention and treatment of high myopia by using scleral reinforcement surgery combined with Chinese medicine therapy. Methods：12 healthy adult rabbits were randomly divided into blank control group, the surgery alone group （scleral reinforcement group）, surgery with tra- ditional Chinese medicine group 1 （posterior scleral reinforcement with myopia Recipe group I） , surgery with traditional Chinese medicine group 2 （ posterior scleral reinforcement with myopia Recipe group II）. Parts of the rabbits were sacri- riced after 12 weeks, picking out the right eye and cutting the sclera attached to graft for the analysis on change of bFGF concentration within postoperative rabbits sclera by cell immunohistochemistry. Results：The bFGF concentration distribu- tion in the blank control group was very sparse with very few proliferation. The initial presentation of surgery alone group had a strong inflammatory response with inflammatory cell infiltration, and the bFGF concentration was sparse with less proliferation. Compared with the surgery alone group, the inflammation of the surgery with Chinese medicine was signifi- cantly lighter with a smaller amount of inflammatory cell infiltration, dense concentration of bFGF and more proliferation. The distribution of concentration of bFGF between surgery with traditional Chinese medicine group 1 and surgery with tra- ditional Chinese medicine group 2 had no significant difference compared with the blank group and the surgery alone group. Conclusion：The posterior scleral reinforcement with Chinese herbal medicine prescription fo

关 键 词：补肾健脾中药 碱性成纤维细胞生长因子(bFGF) 巩膜成纤维细胞 

分 类 号：R285.5[医药卫生—中药学]