慢性乙型肝炎患者病毒前S1抗原与HBV-DNA水平和HBV血清标志物的相关性研究  

Correlation studies of hepatitis B virus preS1-antigen and HBV-DNA,HBV markers in patients with hepatitis B

作　　者：曾钢[1] 李彩东[1] 吴斌[1] 田鹏飞[1] 段正军[1] 

机构地区：[1]兰州市第二人民医院肝病研究所,甘肃兰州730046

出　　处：《现代预防医学》2014年第9期1653-1655,共3页Modern Preventive Medicine

摘　　要：目的探讨慢性乙型肝炎病毒(HBV)感染者血清中前S1抗原(Pre-S1Ag)与HBV-DNA、HBV血清标志物之间的相关性,为临床诊治提供参考。方法采用实时定量聚合酶链反应(Real-Time PCR)方法对血清中HBV-DNA进行检测,同时采用化学发光免疫测定法(CLIA)对HBsAg、Pre-S1Ag和HBeAg进行检测。结果 905例标本中,HBV-DNA阳性率为67.96%(615/905),HBV Pre-S1Ag阳性率为68.51%(620/905),差异无统计学意义(P>0.05);615例HBV-DNA阳性患者中,Pre-S1阳性率80.65%(496/615),显著高于HBV-DNA阴性组的Pre-S1阳性率42.76%(124/290),差异有统计学意义(P<0.01);570例HBeAg阳性组中,HBV Pre-S1阳性率为85.08%(485/570),显著高于HBeAg阴性组的Pre-SAg1阳性率40.30%(135/335),差异有统计学意义(P<0.01)。结论Pre-S1Ag与HBV-DNA阳性相关度高,较HBeAg更敏感,互补性和一致性均较好,可作为判断HBV感染与复制的一项重要检测指标。Objective To investigate the correlations on hepatitis B virus （HBV） preSl-antigen （pre-S1Ag） and HBV-DNA, HBV markers in patients with hepatitis B. Methods The HBsAg, preS1-Ag and HBeAg were determined by CLIA and PCR in 905 pa- tients with hepatitis B and 100 healthy persons. Results The positive rates of HBV-DNA and preS1-Ag in 905 patients were 67.96% （615/905） and 68.51% （620/905）. There was no significant difference between them （P〉0.05）; The positive rates of pre-S1Ag in 615 patients with HBV-DNA positive group were 80.65% （496/615）, significantly higher than that in the HBV-DNA negative group 42.76% （124/290） （P〈0.01）; The positive rate of pre-S1Ag with HBeAg （＋） was 85.08% （485/570）, which was sig- nificantly higher than that in the HBeAg （-） group 40.30% （135/335） （P〈0.01）. Conclusion Pre-S1Ag of HBV is highly related to HBV-DNA. The detection of pre-S1Ag can well reflect the HBV infection and replication.

关 键 词：乙型肝炎病毒 HBV前S1抗原 HBV血清标志物 HBV-DNA 

分 类 号：R115[医药卫生—公共卫生与预防医学]