北京市朝阳区手足口病非EV71、非CoxA16肠道病毒的基因型  

Genetype of non-EV71, non-CoxA16 enteroviruses caused hand, foot and mouth disease in Chaoyang of Beijing

作　　者：王海滨[1] 雷娜[1] 姜晓红[2] 赵剑虹[1] 王晶[2] 宋衍燕[1] 李书明[1] 

机构地区：[1]北京市朝阳区疾病预防控制中心微生物检验科,北京100021 [2]北京市朝阳区疾病预防控制中心流行病科,北京100021

出　　处：《现代预防医学》2014年第9期1699-1703,共5页Modern Preventive Medicine

摘　　要：目的了解2012年北京市朝阳区引起手足口病的非EV71、非CoxAl6型肠道病毒的病原构成及优势型别,为本地区手足口病的防控工作提供科学依据。方法收集2012年手足口病患者咽拭子样本或粪便样本共815份。经Real-time PCR确定非EV71、非CoxAl6型肠道病毒阳性样本;PCR分别扩增病毒5'UTR区和VP2基因区片段,对PCR阳性扩增产物进行测序,经BLAST比对确定病毒型别;并对VP2基因序列进行聚类分析。结果 Real-time PCR确定了61例非EV71、非CoxA16型肠道病毒阳性样本。测序结果共确定CoxA10型44例(73.3%,44/60)、CoxA6型8例(13.3%,8/60)及CoxA4型1例(1.7%,1/60);有1例样本确定为EV71型;另有7例样本分型失败。聚类结果显示本地区的CoxA10型、CoxA6型和CoxA4型肠道病毒的VP2核苷酸序列与中国地区的毒株有较高同源性,而与其各自的原型株同源性相对较低;此外,本地区的CoxA6型肠道病毒存在两个传播链的共循环。结论 2012年北京市朝阳区引起手足口病的非EV71、非CoxAl6型肠道病毒型别多样,包括CoxA10、CoxA6和CoxA4型。CoxAl0型为非EV71、非CoxAl6型肠道病毒中的优势型别。Objective To explore the pathogenic spectrum and predominant type of non-EV71, non-CoxA 16 enteroviruses caused hand, foot and mouth disease （HFMD） in Chaosyang, Beijing in 2012. Methods The 815 specimen^were collected from outpatient and in-patient cases .gf HFMD. The specimens which were non-EV71, non-CoxA16 enteroviruses were identified by real-time PCR. The genotyping of non-EVT1, non-CoxA16 enteroviruses was performed by PCR and sequencing of 5＇UTR and VP2 se- quences. The sequence and phylogenetic analysis of the VP2 genes was performed. Results 61 specimens were identified as non-E^71, non-CoxA16 enterovirus positive by real-time PCR. And the types of the non-EV71, non-CoxA16 enterovirus were i- dentified by sequence BLAST： 44 CoxA10 （73.3%, 44/60）, 8 CoxA6 （13.3%, 8/60） and 1 CoxA4 （1.7%, 1/60）. 1 out of 61 speci- mens was tested as EV71 and 7 specimens were failed to typing. The CoxA10, CoxA6 and CoxA4 enterovirus in this study had high homology with those isolated in China, while lower homology with their prototype viruses described in the NCBI database. Phyloge- netic analysis showed that there were two lineages of CoxA6 co-circulating in Chaoyang. Conclusion Hand, foot and mouth disease in Chaoyang, Beijing is concerned with diverse non-EV71, non-CoxA16 enteroviruses, including CoxA10, CoxA6 and CoxA4. And CoxA10 is the predominant type among non-EV71, non-CoxA16 enteroviruses.

关 键 词：手足口病 肠道病毒 基因型 

分 类 号：R183[医药卫生—流行病学]