罗丹明6G荧光猝灭法测定痕量肝素  

Fluorescence quenching method for determination of trace heparin with rhodamine 6G system

作　　者：吴雯辉[1] 董存智[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,淮北235000

出　　处：《分析试验室》2014年第5期601-603,共3页Chinese Journal of Analysis Laboratory

摘　　要：在pH8．0的缓冲体系中，肝素使得罗丹明6G的荧光发生明显猝灭，罗丹明6G的荧光猝灭程度在一定范围内与肝素的浓度成正比，据此建立了荧光猝灭法测定痕量肝素的方法，并对体系的荧光猝灭机理进行了讨论。体系的激发波长为482nm，发射波长为560nm，线性范围为0．05—3．0mg／L，检出限为6．1μg/L。方法已应用于肝素钠注射液中肝素含量的测定。Heparin could react with rhodamine 6G in the buffer solution at pH 8.0 to form a complex, which generates obvious fluorescence quenching phenomenon. Based on this, a new spectrofluorimetric method was developed for the determination of trace amounts of heparin. Its maximum excitation wavelength is at 482 nm, and the maximum emission wavelength is at 560 nm. The linear range and detection limit for the determination of heparin were 0. 05 to 3.0 mg/L and 0. 0061 mg/L, respectively. This method is simple, practical, and it has been applied successfully to assessment of heparin in real samples with satisfactory results. Moreover, the quenching mechanism of the system has also been preliminary discussed.

关 键 词：罗丹明6G 肝素 荧光猝灭 

分 类 号：O657.3[理学—分析化学]