小麦全蚀病生防芽孢杆菌产芽孢培养基的响应面法优化  

Optimization of Spores Production Culture Medium of Biocontrol Bacillus Bacteria against Wheat Take-all Diseases Using Response Surface Method

作　　者：张冬冬[1] 高同国[1] 朱宝成[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《麦类作物学报》2014年第3期418-424,共7页Journal of Triticeae Crops

基　　金：河北省科学技术研究与发展计划项目(11220313)

摘　　要：为了优化小麦全蚀病生防芽孢杆菌Z-14菌株产芽孢培养基,通过Plackett-Burman实验分析影响芽孢产量的主要碳源、氮源和无机盐,用最陡爬坡路径逼近最大响应区域,利用Box-Behnken实验设计及响应面分析法筛选主要影响因素的最佳水平。结果表明,Z-14菌株生产芽孢最适碳源、无机盐和氮源分别为玉米粉、MnSO4·H2O和酵母粉。优化培养基组合为玉米粉1.17%、酵母粉3.31%、MnSO4·H2O 0.072%、NaH2PO4·2H2O 0.2%、Na2HPO4·2H2O 0.4%,优化后芽孢产量达到1.85×109 cfu·mL-1,与理论值基本相符。In order to optimize the spores production culture medium of biocontrol Bacillus bacteria Z- 14 strain against wheat take-all diseases. Plackett-Burman experimental design was used to determine the main factors of carbon source, nitrogen source and inorganic salt. The pass of steepest ascent was undertaken to approach the optimal response region of the significant factors. Box-Behnken design and response surface analysis were adopted to further investigate the mutual interaction between the varia- bles and to identify the optimal values that bring maximum spore yield. The result showed that corn flour,MnSO4 ~ H20 and soybean meal were the optimum carbon source, inorganic salt and nitrogen source based on Plackett-Burman design, respectively. The optimal culture medium was as followed： corn flour 1. 17%, yeast 3. 31%, MnSO4 · H20 0. 072%, NaH2PO4· 2H2O 0. 2%, Na2HP 2H20 0.4%. The spore yield reached 1.85× 109 cfu · mL-1 after fermentation experiment under mal condition and basically consistent with the theoretical value. Response surface method can be to optimize the spores production culture medium of Z-14 strain. 04 Q opti- used

关 键 词：小麦全蚀病 生物防治 芽孢杆菌 产芽孢培养基 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]