甲嘎松汤提取工艺的正交设计法优选  

Studies on optimum extraction technology for JiaGaSong Soup by orthogonal design

作　　者：赵玉民[1] 廖嘉仪[1] 熊天琴[1] 康萨.索朗其美 泽仁达瓦[2] 何国林[1] 桑传兰[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]西藏藏医学院,西藏拉萨850000

出　　处：《时珍国医国药》2014年第4期769-771,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81202997);广东省科技计划项目(No.2011B031700075);广东省自然科学基金(No.S2011010003925)

摘　　要：目的建立同时测定甲嘎松汤中两种成分含量的高效液相色谱法,采用正交设计法优选甲嘎松汤中有效成分的提取工艺。方法以6-姜辣素和去氢二异丁香酚的总含量为指标,采用正交实验,对提取时间、乙醇体积分数及乙醇用量进行考察,优化提取条件。结果最佳提取工艺条件为加30倍90%的乙醇,超声30 min。结论该提取工艺合理,有效成分提取效率较高。Objective To establish an HPLC method for simultaneous determination of two kinds of JiaGaSong Soup ingredients and optimize extraction process of JiaCaSong Soup by Orthogonal Design. Methods Using the total content of 6 - Cingerol and De- hydrodiisoeugenol as indicators to study the extraction time, the volume fraction of ethanol by orthogonal experiment , thus we can optimize the extraction condition. Results Optimum extraction technology was that added 30 times of 90% ethanol, ultrasonic 30 min. Conclusion The optimum extraction technology was reasonable and the extraction for effective component was of high effi- ciency.

关 键 词：甲嘎松汤 6-姜辣素 去氢二异丁香酚 正交设计 高效液相色谱 

分 类 号：R291.408[医药卫生—民族医学]