参苓白术散通过MAPK信号通路对炎症性肠炎小鼠的作用  

Effect of Shenling Baizhu San through the Pathway of MAPK on the Murine Model of IBD Induced by DSS in Mice

作　　者：刘玉晖[1] 严雪梅[1] 佟阳[1] 易文凤 胡婕[1] 游宇[2] 

机构地区：[1]江西中医药大学药学院,江西南昌330004 [2]南昌大学第一附属医院,江西南昌330006

出　　处：《时珍国医国药》2014年第4期793-795,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(No.81160540/H2814)

摘　　要：目的探讨参苓白术散通过ERK/P38MAPK信号通路对DSS诱导的IBD(炎症性肠炎)的作用。方法 5%葡聚糖硫酸钠(dextran sodium sulphate,DSS)自由饮水7 d诱导BLALB/c小鼠急性炎症性肠病,同时各组小鼠予以生理盐水、柳氮磺胺吡啶、参苓白术散高、中、低剂量(12,6,3 g/kg)灌胃,采用RT-PCR及Western Blot检测各组动物肠道组织中ERK、P38MAPK的表达情况。结果小鼠结肠组织中ERK及p38MAPK水平模型组高于正常对照组,3种不同剂量的参林白术散对其表达有相应的下调。结论参苓白术散通过ERK/P38MAPK信号通路作用而起到抗DSS诱导的IBD的作用。Objective To study the effect of Shenling Baizhu San through the pathway of ERK/p38MAPK on the Murine Model of IBD induced by DSS in mice. Methods IBD was induced by taking the drinking water added 5% dextran sodium sulphate （DSS） for 7 days. At the same time, the BALB/c mice of different groups were ig administrated with normal sodium,Sulfasalazine ,Shen- ling Baizhu San （ 12,6,3 g · kg^-1 ） ,respectively. The effects on expression changes of ERK and p38MAPK in model rats were detected by RT - PCR and Western blot. Results Levels of ERK and p38MAPK in DSS induced group were increased , Three doses of Shenling Baizhu San could down - regulate the expression. Conclusion Shenling Baizhu San can protect the damage in DSS - induced IBD through the Pathway of ERK/P38MAPK .

关 键 词：参苓白术散 炎症性肠炎 MAPK 

分 类 号：R285.5[医药卫生—中药学]