1种适用于转基因玉米PCR检测的DNA快速提取方法  

A Rapid DNA Extraction Method Applying to PCR Detection of Transgenic Maize

作　　者：戴军[1,2] 汪海[2] 朱莉[2] 黄大昉[2] 郎志宏[2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《安徽农业科学》2014年第12期3494-3496,共3页Journal of Anhui Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(批准号:2013ZX08003-001);国家自然科学基金(批准号:30970231)

摘　　要：[目的]建立一种快速提取玉米基因组DNA的方法。[方法]对改良CTAB法进行进一步简化,不需要进行沉淀、洗涤、溶解和RNA消化等步骤,建立一种快速提取玉米基因组DNA的方法。[结果]该方法获得的DNA完整性及PCR扩增效果与试剂盒提取方法相比无明显差异;经过对样品ELISA检验可知,该方法得到的DNA用于PCR检测结果准确。[结论]该方法能够满足转基因玉米PCR检测中快速、高效、准确、高通量的要求。[ Objective ] To establish a fast method to extract maize genome DNA. [ Method ] Improved CTAB method was further simplified, a method for rapid extraction of the maize genome DNA was established without the steps of alcohol precipitation, washing, dissolving and RNA digestion. [ Result] The DNA integrity and the PCR amplification using this method showed no significant difference from the plant ge- nomic DNA extraction kit method. Furthermore, it was same detection results with ELISA analysis and with PCR detection using DNA template obtained by this method. [ Conclusion] Therefore, this method is a rapid, efficient, accurate, high-throughput method that meets the PCR de- tection requirements of genetically modified maize.

关 键 词：转基因玉米 基因组DNA 快速提取 PCR检测 

分 类 号：S513[农业科学—作物学]