超临界CO_2流体萃取南方红豆杉叶中脂肪油的工艺研究及其成分分析  

Supercritical CO_2 Fluid Extraction and Component Analysis of Leaves Oil from Taxus chinensis var. mairei

作　　者：汤彬[1] 张奉苏[1] 李祥[1] 陈建伟[1] 姚晓[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023

出　　处：《中药材》2013年第12期2023-2027,共5页Journal of Chinese Medicinal Materials

基　　金：江苏高校优势学科建设工程资助项目(ysxk-2010);南京中医药大学中药学一级学科开放课题资助(2011ZYX6-008)

摘　　要：目的:研究超临界CO2流体萃取南方红豆杉叶中脂肪油的最佳工艺及其成分分析。方法:以南方红豆杉叶脂肪油萃取得率为评价指标,考察不同萃取压力、萃取温度、萃取时间对脂肪油萃取率的影响,并采用衍生化GC-MS法对南方红豆杉叶脂肪油进行成分分析。结果:超临界CO2萃取南方红豆杉叶脂肪油最终优化的萃取工艺为:CO2压缩泵频率为10 Hz,萃取压力为25 MPa,萃取温度为45℃,萃取时间为120 min,分离釜Ⅰ压力8.0 MPa、温度40℃,分离釜Ⅱ压力5.0 MPa、温度35℃。萃取得到的脂肪油经三氟化硼衍生化后,经GC-MS分析,共鉴定出了33种脂肪酸。结论:3种不同产地的南方红豆杉叶脂肪油的萃取率不同,且以无锡东港的萃取率最高为2.61%。对其成分的分析,不同产地和不同提取方法下主要脂肪酸种类基本一致,含量较低的脂肪酸种类差别较大。Objective： To research the optimal extraction process of supercritical CO2extraction and analyze the component of the oil extracted from leaves of Taxus chinensis var. mairei. Methods： Using the yield of leaves oil from Taxus chinensis var. mairei as the index,investigated the effect of the extraction pressure,extraction temperature and extraction time on the extracting-rate of leaves oil. The chemical composition of the extracted leaves oil was analyzed by derivatized GC-MS. Results： The optimal parameters of the supercritical CO2extraction of the oil extracted from leaves of Taxus chinensis var. mairei were determined： CO2compressor pump frequency was 10 Hz,the extraction pressure was 25 MPa and the temperature of extraction was 45 ℃,the extraction time was 120 min,the isolatorⅠpressure was 8. 0 MPa and the temperature of extraction was 40 ℃,the isolatorⅡpressure was 5. 0 MPa and the temperature of extraction was 35 ℃. The extracted leaves oil was derivatized with boron trifluoride-methanol complex. Thirty-three kinds of fatty acids were identified by GC-MS. Conclusion： The yield of leaves oils are different from Taxus chinensis var. mairei from 3 habitats. The yield of leaves oil from Donggang,Wuxi city is the highest,about 2. 61%. The kinds of fatty acids with high amounts in leaves oil from Taxus chinensis var. mairei is identical in general,the kinds of fatty acids with low amounts in leaves oil from Taxus chinensis var. mairei have differences.

关 键 词：GC-MS分析 超临界CO2萃取 脂肪酸 甲酯化 南方红豆杉 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]