RNAi沉默LIMK2基因对成骨细胞COX-2基因力学敏感性的影响  

Effects of LIMK2 RNA interference on the mechanosensitivity of COX-2 gene in osteoblasts

作　　者：付强[1] 杨志[1] 吴昌敬 陈晓丹[1] 谭淑仪[1] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院.广东省口腔医学重点实验,副主任医师广东510055 [2]广州市中西医结合医院,主治医师硕士广东510800

出　　处：《中华老年口腔医学杂志》2013年第6期325-329,共5页Chinese Journal of Geriatric Dentistry

基　　金：广东省科技计划项目(编号:2012B031800093)

摘　　要：目的:采用RNAi技术沉默LIMK2基因来抑制细胞骨架改建,观察其对流体剪切力下成骨细胞COX-2基因力学敏感性的影响。方法;RNAi技术沉默原代小鼠成骨细胞LIMK2基因来抑制细胞骨架改建,分别用实时荧光定量巢式PCR和免疫荧光的方法检测流体剪切力下COX-2基因mRNA和蛋白的表达水平。结果:RNAi抑制细胞骨架改建可以促进流体剪切力诱导的成骨细胞COX-2 mRNA和蛋白的表达(P<0.05)。结论:采用RNAi技术抑制细胞骨架改建,可以提高流体剪切力作用下成骨细胞COX-2基因的力学敏感性。Objective： To study the effect of cytoskelet on reorganization in hibition with RNA interference on the mechanosensitivity of COX -2 gene in osteoblasts induced by fluid shear stress.Methods： RNA interference against LIMK2 gene was used to inhibit the cytoskeleton reorganization of BALB/cmouse prim ary osteoblasts.Osteoblasts were loaded by fluid shear stress at12 dyne/cm 2 or unloaded.The nested real-time PCR and immun of luorescence were performed to detect the expression levels of COX-2m RNA and protein, respectively. Result： Cytoskeleton reorganization inhibition with RNA interference against LIMK2 gene increased the expression of COX -2 gene induced by fluid shear stress.Conclusion： Cytoskeleton reorganization inhibition with RNA interference against LIMK2 gene could enhance the mechanosensitivity of COX -2 gene induced by fluid shearstress.

关 键 词：成骨细胞 细胞骨架 流体剪切力 COX-2基因 细胞力学敏感性 

分 类 号：R318.01[医药卫生—生物医学工程]